half_strenghs_hyphomicrobium_medium

CultureMech:000884 bacterial COMPLEX SOLID_AGAR
Original name: HALF STRENGHS HYPHOMICROBIUM MEDIUM

Overview

External ontology
mediadive.medium:1420 (source link unavailable) — HALF STRENGHS HYPHOMICROBIUM MEDIUM
Applications
  • Microbial cultivation
pH
7.5
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1420.pdf

Composition

flowchart LR
    CultureMech_000884["half_strenghs_hyphomicrobium_medium"]
    ing0_Vitamin_B12["Vitamin B12"]
    CultureMech_000884 --> ing0_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing0_Vitamin_B12 -.-> CHEBI_176843
    ing1_KH2PO4["KH2PO4"]
    CultureMech_000884 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
    CultureMech_000884 --> ing2_Na2HPO4_x_2_H2O
    CHEBI_91258["CHEBI:91258"]
    ing2_Na2HPO4_x_2_H2O -.-> CHEBI_91258
    ing3_Methylamine_hydrochloride["Methylamine hydrochloride"]
    CultureMech_000884 --> ing3_Methylamine_hydrochloride
    CHEBI_59337["CHEBI:59337"]
    ing3_Methylamine_hydrochloride -.-> CHEBI_59337
    ing4__NH4_2SO4["(NH4)2SO4"]
    CultureMech_000884 --> ing4__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing4__NH4_2SO4 -.-> CHEBI_62946
    ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000884 --> ing5_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing6_Bacto_peptone["Bacto peptone"]
    CultureMech_000884 --> ing6_Bacto_peptone
    ing7_Agar["Agar"]
    CultureMech_000884 --> ing7_Agar
    CHEBI_2509["CHEBI:2509"]
    ing7_Agar -.-> CHEBI_2509
    ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000884 --> ing8_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing9_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000884 --> ing9_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing9_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing10_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_000884 --> ing10_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing10_MnSO4_x_H2O -.-> CHEBI_86364
    ing11_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000884 --> ing11_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing11_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing12_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_000884 --> ing12_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing12_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing13__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
    CultureMech_000884 --> ing13__NH4_6Mo7O24_x_4_H2O
    CHEBI_91249["CHEBI:91249"]
    ing13__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
    ing14_Na_EDTA["Na-EDTA"]
    CultureMech_000884 --> ing14_Na_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing14_Na_EDTA -.-> CHEBI_64734
    ing15_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000884 --> ing15_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing15_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing16_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000884 --> ing16_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing16_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing17_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
    CultureMech_000884 --> ing17_Co_NO3_2_x_6_H2O
    CHEBI_86214["CHEBI:86214"]
    ing17_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
    ing18_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_000884 --> ing18_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing18_Na2B4O7_x_10_H2O -.-> CHEBI_131366
    ing19_Biotin["Biotin"]
    CultureMech_000884 --> ing19_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing19_Biotin -.-> CHEBI_15956
    ing20_Folic_acid["Folic acid"]
    CultureMech_000884 --> ing20_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing20_Folic_acid -.-> CHEBI_27470
    ing21_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_000884 --> ing21_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing21_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing22_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_000884 --> ing22_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing22_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing23_Riboflavin["Riboflavin"]
    CultureMech_000884 --> ing23_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing23_Riboflavin -.-> CHEBI_17015
    ing24_Nicotinic_acid["Nicotinic acid"]
    CultureMech_000884 --> ing24_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing24_Nicotinic_acid -.-> CHEBI_15940
    ing25_D_Calcium_pantothenate["D-Calcium pantothenate"]
    CultureMech_000884 --> ing25_D_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing25_D_Calcium_pantothenate -.-> CHEBI_31345
    ing26_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_000884 --> ing26_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing26_p_Aminobenzoic_acid -.-> CHEBI_30753

Ingredients (27)

# Ingredient Concentration Ontology Notes
1 Vitamin B12 0.00010250000000000001 G_PER_L CHEBI:176843
vitamin B12
 [Merged 3 duplicates: 1.25e-06, 1.25e-06, 0.0001]
2 KH2PO4
H2O4P.K 136.084 g/mol
13 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
26.8 G_PER_L CHEBI:91258
disodium hydrogenphosphate dihydrate
4 Methylamine hydrochloride
CH6N.Cl 67.519 g/mol
33.8 G_PER_L CHEBI:59337
methylamine hydrochloride
5 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.25 G_PER_L CHEBI:62946
ammonium sulfate
6 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
29.8 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 0.1, 29.7]
7 Bacto peptone 0.125 G_PER_L
8 Agar 15 G_PER_L CHEBI:2509
agar
 (for solid medium)
9 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
5.325 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 1.99, 3.335]
10 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
1.599 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 [Merged 3 duplicates: 1.0, 0.099, 0.5]
11 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.504 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
 [Merged 2 duplicates: 0.35, 0.154]
12 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.5 G_PER_L CHEBI:75213
sodium molybdate dihydrate
13 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
10 G_PER_L CHEBI:44557
nitrilotriacetic acid
14 (NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
0.00925 G_PER_L CHEBI:91249
ammonium molybdate
15 Na-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.25 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
16 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
1.095 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
17 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.0392 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
18 Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
0.0248 G_PER_L CHEBI:86214
cobalt dinitrate hexahydrate
19 Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
0.0177 G_PER_L CHEBI:131366
disodium tetraborate decahydrate
20 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
21 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
22 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
23 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.005 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
24 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
25 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
26 D-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
27 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid

Preparation

  1. For solid media add 15.0 g agar to solution B
  2. Autoclave solution A and B separately and combine after cooling.
  3. Prepare the liquid medium. Before inoculation add from sterile stock solutions:
  4. Adjust pH to 7.5
  5. Adjust pH to 7.5
  6. Dissolve the nitrilotriacetic acid.
  7. Adjust pH to 7.0 with KOH (~7.3 g).
  8. Dissolve other salts separately and combine.
  9. Adjust pH to 6.8 with NaOH or H2SO4.
  10. Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.372401Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1420
2026-01-28T19:48:09.481121Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T03:08:42.546387Z data-quality-cleanup-v1.0 Data quality fixes Merged 7 duplicate ingredient(s)
2026-03-13T06:18:59.214387Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000884
2026-03-17T06:29:45.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:10.703045+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HALF STRENGHS HYPHOMICROBIUM MEDIUM" to "half_strenghs_hyphomicrobium_medium" for consistent matching
2026-06-05T07:41:07.152558+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 24 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.312874+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.