alteromonas_medium

CultureMech:000896 bacterial DEFINED SOLID_AGAR
Original name: ALTEROMONAS MEDIUM

Overview

External ontology
mediadive.medium:1434 (source link unavailable) — ALTEROMONAS MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1434.pdf

Composition

flowchart LR
    CultureMech_000896["alteromonas_medium"]
    ing0__NH4_2SO4["(NH4)2SO4"]
    CultureMech_000896 --> ing0__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing0__NH4_2SO4 -.-> CHEBI_62946
    ing1_K2HPO4["K2HPO4"]
    CultureMech_000896 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_KH2PO4["KH2PO4"]
    CultureMech_000896 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_NaCl["NaCl"]
    CultureMech_000896 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000896 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_Methanol["Methanol"]
    CultureMech_000896 --> ing5_Methanol
    CHEBI_17790["CHEBI:17790"]
    ing5_Methanol -.-> CHEBI_17790
    ing6_Agar["Agar"]
    CultureMech_000896 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509
    ing7_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_000896 --> ing7_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing7_MnSO4_x_H2O -.-> CHEBI_86364
    ing8_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000896 --> ing8_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing8_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing9_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000896 --> ing9_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing9_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing10_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000896 --> ing10_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing10_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing11_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000896 --> ing11_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing11_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000896 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000896 --> ing13_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing13_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing14_Vitamin_B12["Vitamin B12"]
    CultureMech_000896 --> ing14_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing14_Vitamin_B12 -.-> CHEBI_176843

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 (NH4)2SO4
2H4N.O4S 132.141 g/mol
2 G_PER_L CHEBI:62946
ammonium sulfate
2 K2HPO4
HO4P.2K 174.174 g/mol
2 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 KH2PO4
H2O4P.K 136.084 g/mol
1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
4 NaCl
Cl.Na 58.443 g/mol
20 G_PER_L CHEBI:26710
sodium chloride
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.3 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 Methanol
CH4O 32.042 g/mol
7.92 G_PER_L CHEBI:17790
methanol
7 Agar 15 G_PER_L CHEBI:2509
agar
8 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.76 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
9 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.25 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
10 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.28 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
11 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
1.4 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
12 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.15 G_PER_L CHEBI:86158
calcium chloride dihydrate
13 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.24 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
14 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.24 G_PER_L CHEBI:75213
sodium molybdate dihydrate
15 Vitamin B12 0.001 G_PER_L CHEBI:176843
vitamin B12

Preparation

  1. pH 7.0
  2. Prepare the medium without methanol and vitamin B12 in closed vessels with a medium to headspace ratio of 1:5 to 1:10. Adjust pH to 7.0 and sterilize. Allow to cool to about 45°C before adding 1mL filter-sterilized Vitamin B12 stock solution (0.1mg/100ml) and 10 mL filter-sterilized methanol per litre medium.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.408053Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1434
2026-03-13T06:18:59.313239Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000896
2026-03-17T06:30:20.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:10.815854+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ALTEROMONAS MEDIUM" to "alteromonas_medium" for consistent matching
2026-06-05T07:40:48.323909+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:52.962123+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.