pirellula_medium_m31_py

CultureMech:000900 bacterial COMPLEX SOLID_AGAR
Original name: PIRELLULA MEDIUM (M31 PY)

Overview

External ontology
mediadive.medium:1438 (source link unavailable) — PIRELLULA MEDIUM (M31 PY)
Applications
  • Microbial cultivation
pH range
{'min': 7.5, 'max': 8.5}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1438.pdf

Composition

flowchart LR
    CultureMech_000900["pirellula_medium_m31_py"]
    ing0_Peptone["Peptone"]
    CultureMech_000900 --> ing0_Peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_000900 --> ing1_Yeast_extract
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000900 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000900 --> ing3_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing4_Tris_HCl_buffer["Tris-HCl buffer"]
    CultureMech_000900 --> ing4_Tris_HCl_buffer
    CHEBI_9754["CHEBI:9754"]
    ing4_Tris_HCl_buffer -.-> CHEBI_9754
    ing5_Agar["Agar"]
    CultureMech_000900 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509
    ing6_N_Acetylglucosamine["N-Acetylglucosamine"]
    CultureMech_000900 --> ing6_N_Acetylglucosamine
    CHEBI_59640["CHEBI:59640"]
    ing6_N_Acetylglucosamine -.-> CHEBI_59640
    ing7_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
    CultureMech_000900 --> ing7_Na2HPO4_x_2_H2O
    CHEBI_91258["CHEBI:91258"]
    ing7_Na2HPO4_x_2_H2O -.-> CHEBI_91258
    ing8_Vitamin_B12["Vitamin B12"]
    CultureMech_000900 --> ing8_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing8_Vitamin_B12 -.-> CHEBI_176843
    ing9_Biotin["Biotin"]
    CultureMech_000900 --> ing9_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing9_Biotin -.-> CHEBI_15956
    ing10_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_000900 --> ing10_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing10_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing11_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_000900 --> ing11_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing11_Calcium_pantothenate -.-> CHEBI_31345
    ing12_Folic_acid["Folic acid"]
    CultureMech_000900 --> ing12_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing12_Folic_acid -.-> CHEBI_27470
    ing13_Riboflavin["Riboflavin"]
    CultureMech_000900 --> ing13_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing13_Riboflavin -.-> CHEBI_17015
    ing14_Nicotinamide["Nicotinamide"]
    CultureMech_000900 --> ing14_Nicotinamide
    CHEBI_17154["CHEBI:17154"]
    ing14_Nicotinamide -.-> CHEBI_17154
    ing15_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_000900 --> ing15_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing15_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing16_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_000900 --> ing16_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing16_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing17_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_000900 --> ing17_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing17_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing18_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000900 --> ing18_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing18_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing19__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
    CultureMech_000900 --> ing19__NH4_6Mo7O24_x_4_H2O
    CHEBI_91249["CHEBI:91249"]
    ing19__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
    ing20_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000900 --> ing20_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing20_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing21_Na_EDTA["Na-EDTA"]
    CultureMech_000900 --> ing21_Na_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing21_Na_EDTA -.-> CHEBI_64734
    ing22_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000900 --> ing22_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing22_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing23_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_000900 --> ing23_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing23_MnSO4_x_H2O -.-> CHEBI_86364
    ing24_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000900 --> ing24_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing24_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing25_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
    CultureMech_000900 --> ing25_Co_NO3_2_x_6_H2O
    CHEBI_86214["CHEBI:86214"]
    ing25_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
    ing26_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_000900 --> ing26_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing26_Na2B4O7_x_10_H2O -.-> CHEBI_131366

Ingredients (27)

# Ingredient Concentration Ontology Notes
1 Peptone 0.263158 G_PER_L
2 Yeast extract 0.263158 G_PER_L
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
3.440263 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.105263, 3.335]
4 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.105263 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
5 Tris-HCl buffer
C4H11NO3 121.136 g/mol
50 G_PER_L CHEBI:9754
tris
6 Agar 18.9474 G_PER_L CHEBI:2509
agar
7 N-Acetylglucosamine 40 G_PER_L CHEBI:59640
N-acetylglucosamine
8 Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
2 G_PER_L CHEBI:91258
disodium hydrogenphosphate dihydrate
9 Vitamin B12 0.0002 G_PER_L CHEBI:176843
vitamin B12
10 Biotin
C10H16N2O3S 244.316 g/mol
0.004 G_PER_L CHEBI:15956
biotin
11 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.01 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
12 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.01 G_PER_L CHEBI:31345
Calcium pantothenate
13 Folic acid
C19H19N7O6 441.404 g/mol
0.004 G_PER_L CHEBI:27470
folic acid
14 Riboflavin
C17H20N4O6 376.369 g/mol
0.01 G_PER_L CHEBI:17015
riboflavin
15 Nicotinamide
C6H6N2O 122.127 g/mol
0.01 G_PER_L CHEBI:17154
nicotinamide
16 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.01 G_PER_L CHEBI:30753
4-aminobenzoic acid
17 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.02 G_PER_L CHEBI:30961
pyridoxine hydrochloride
18 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
10 G_PER_L CHEBI:44557
nitrilotriacetic acid
19 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
29.7 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
20 (NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
0.00925 G_PER_L CHEBI:91249
ammonium molybdate
21 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.599 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 [Merged 2 duplicates: 0.099, 0.5]
22 Na-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.25 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
23 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
1.095 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
24 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.154 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
25 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.0392 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
26 Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
0.0248 G_PER_L CHEBI:86214
cobalt dinitrate hexahydrate
27 Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
0.0177 G_PER_L CHEBI:131366
disodium tetraborate decahydrate

Preparation

  1. After autoclaving add solution 2 to solution 1.
  2. Adjust pH to 7.5, autoclave at 121°C for 20 min.
  3. For agar plates increase the pH of the Tris/HCl buffer to 8.5.
  4. Adjust pH to 7.5, filter-sterilize
  5. filter-sterilized
  6. Dissolve the nitrilotriacetic acid.
  7. Adjust pH to 7.0 with KOH (~7.3 g).
  8. Dissolve other salts separately and combine.
  9. Adjust pH to 6.8 with NaOH or H2SO4.
  10. Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.421780Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1438
2026-03-13T03:08:41.379977Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:18:59.357881Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000900
2026-03-17T06:29:43.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:10.863914+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PIRELLULA MEDIUM (M31 PY)" to "pirellula_medium_m31_py" for consistent matching
2026-05-20T06:02:02.563245+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7.5-8.5' -> {'min': 7.5, 'max': 8.5}
2026-06-05T07:41:41.959100+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:10.248946+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.