alkalispirillum_mobile_medium

CultureMech:000909 bacterial COMPLEX SOLID_AGAR
Original name: ALKALISPIRILLUM MOBILE MEDIUM

Overview

External ontology
mediadive.medium:1446 (source link unavailable) — ALKALISPIRILLUM MOBILE MEDIUM
Applications
  • Microbial cultivation
pH
9.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1446.pdf

Composition

flowchart LR
    CultureMech_000909["alkalispirillum_mobile_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_000909 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_Na2S2O3["Na2S2O3"]
    CultureMech_000909 --> ing1_Na2S2O3
    CHEBI_132112["CHEBI:132112"]
    ing1_Na2S2O3 -.-> CHEBI_132112
    ing2_KH2PO4["KH2PO4"]
    CultureMech_000909 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_Na_acetate["Na-acetate"]
    CultureMech_000909 --> ing3_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing3_Na_acetate -.-> CHEBI_32954
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_000909 --> ing4_Yeast_extract
    ing5_MgCl2_x_7_H2O["MgCl2 x 7 H2O"]
    CultureMech_000909 --> ing5_MgCl2_x_7_H2O
    CHEBI_6636["CHEBI:6636"]
    ing5_MgCl2_x_7_H2O -.-> CHEBI_6636
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000909 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_NH4Cl["NH4Cl"]
    CultureMech_000909 --> ing7_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing7_NH4Cl -.-> CHEBI_31206
    ing8_Na2CO3["Na2CO3"]
    CultureMech_000909 --> ing8_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing8_Na2CO3 -.-> CHEBI_29377
    ing9_NaHCO3["NaHCO3"]
    CultureMech_000909 --> ing9_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing9_NaHCO3 -.-> CHEBI_32139
    ing10_Agar["Agar"]
    CultureMech_000909 --> ing10_Agar
    CHEBI_2509["CHEBI:2509"]
    ing10_Agar -.-> CHEBI_2509
    ing11_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_000909 --> ing11_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing11_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000909 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000909 --> ing13_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing13_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000909 --> ing14_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing15_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000909 --> ing15_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing15_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing16_ZnCl2["ZnCl2"]
    CultureMech_000909 --> ing16_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing16_ZnCl2 -.-> CHEBI_49976
    ing17_H3BO3["H3BO3"]
    CultureMech_000909 --> ing17_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing17_H3BO3 -.-> CHEBI_33118
    ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000909 --> ing18_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing19_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_000909 --> ing19_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing19_Na2SeO3_x_5_H2O -.-> CHEBI_131361

Ingredients (20)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
20 G_PER_L CHEBI:26710
sodium chloride
2 Na2S2O3
2Na.O3S2 158.111 g/mol
1 G_PER_L CHEBI:132112
sodium thiosulfate
3 KH2PO4
H2O4P.K 136.084 g/mol
0.8 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
4 Na-acetate
C2H3O2.Na 82.034 g/mol
2 G_PER_L CHEBI:32954
sodium acetate
5 Yeast extract 1 G_PER_L
6 MgCl2 x 7 H2O
2Cl.Mg 95.211 g/mol
0.1 G_PER_L CHEBI:6636
magnesium dichloride
7 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.05 G_PER_L CHEBI:86158
calcium chloride dihydrate
8 NH4Cl
Cl.H4N 53.492 g/mol
0.8 G_PER_L CHEBI:31206
ammonium chloride
9 Na2CO3
CO3.2Na 105.988 g/mol
21.2 G_PER_L CHEBI:29377
sodium carbonate
10 NaHCO3
CHO3.Na 84.006 g/mol
13.44 G_PER_L CHEBI:32139
sodium hydrogencarbonate
11 Agar 20 G_PER_L CHEBI:2509
agar
 (for solid medium)
12 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.8 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
13 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.25 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
14 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.01 G_PER_L CHEBI:34887
nickel dichloride
15 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
16 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.07 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
17 ZnCl2
2Cl.Zn 136.296 g/mol
0.1 G_PER_L CHEBI:49976
zinc dichloride
18 H3BO3
H3BO3 61.833 g/mol
0.5 G_PER_L CHEBI:33118
boric acid
19 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate
20 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.01 G_PER_L CHEBI:131361
disodium selenite pentahydrate

Preparation

  1. We recommend that liquid and solid media be made by autoclaving the Na2CO3 separately, otherwise the high pH will cause ammonia to be lost. When making media solidified with agar (20 g/l) the agar should also not be autoclaved with the Na2CO3 otherwise the agar will be hydrolysed. When mixing the ingredients for media cool all components to about 55°C otherwise hydrolysis of the agar may occur. Adjust pH after autoclaving by adding the NaHCO3 (8 %) to pH 9.0. Please note that the NaHCO3 solution should be filter sterilized.
  2. For dissolving adjust pH to about 3 with 1 N HCl.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.458049Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1446
2026-01-28T19:48:16.587780Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:18:59.471874Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000909
2026-03-17T06:30:00.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:10.985848+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ALKALISPIRILLUM MOBILE MEDIUM" to "alkalispirillum_mobile_medium" for consistent matching
2026-06-05T07:40:48.215178+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 18 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:34.501338+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:46:52.918434+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.