Overview
- External ontology
- mediadive.medium:1453 (source link unavailable) — ALTERYTHROBACTER MEDIUM 216L
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1453.pdf
Composition
flowchart LR
CultureMech_000919["alterythrobacter_medium_216l"]
ing0_Sodium_acetate["Sodium acetate"]
CultureMech_000919 --> ing0_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing0_Sodium_acetate -.-> CHEBI_32954
ing1_Tryptone["Tryptone"]
CultureMech_000919 --> ing1_Tryptone
ing2_Yeast_extract["Yeast extract"]
CultureMech_000919 --> ing2_Yeast_extract
ing3_Sodium_citrate["Sodium citrate"]
CultureMech_000919 --> ing3_Sodium_citrate
CHEBI_53258["CHEBI:53258"]
ing3_Sodium_citrate -.-> CHEBI_53258
ing4_NH4NO3["NH4NO3"]
CultureMech_000919 --> ing4_NH4NO3
CHEBI_63038["CHEBI:63038"]
ing4_NH4NO3 -.-> CHEBI_63038
ing5_Agar["Agar"]
CultureMech_000919 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
ing6_NaCl["NaCl"]
CultureMech_000919 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
1 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 2 | Tryptone | 10 G_PER_L | ||
| 3 | Yeast extract | 2 G_PER_L | ||
| 4 |
Sodium citrate
C6H5O7.3Na 258.069 g/mol
|
0.5 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 5 |
NH4NO3
H4N.NO3 80.043 g/mol
|
0.2 G_PER_L |
CHEBI:63038
ammonium nitrate
|
|
| 6 | Agar | 12 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
20 G_PER_L |
CHEBI:26710
sodium chloride
|
Preparation
- Adjust to pH 7.5 and sterilize by autoclaving. The medium may be solidified by adding 12.0 g/l agar.
- Note: alternatively, distilled water can be replaced by seawater if the NaCl is omitted.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.476566Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1453 |
| 2026-01-28T19:48:37.212869Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:18:59.563778Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000919 |
| 2026-03-17T06:30:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:11.087132+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ALTERYTHROBACTER MEDIUM 216L" to "alterythrobacter_medium_216l" for consistent matching |
| 2026-06-05T07:40:48.331075+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.965969+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |