alternative_rumen_bacteria_medium

Overview

External ontology

mediadive.medium:1455 (source link unavailable) — ALTERNATIVE RUMEN BACTERIA MEDIUM

Applications

• Microbial cultivation

pH range

{'min': 6.6, 'max': 6.8}

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1455.pdf Commercial Product: Brain Heart Infusion Supplier: Difco/BD Catalog: 237500 Source: https://microbenotes.com/brain-heart-infusion-bhi-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification. Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_000921["alternative_rumen_bacteria_medium"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_000921 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_KH2PO4["KH2PO4"]
    CultureMech_000921 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_NaCl["NaCl"]
    CultureMech_000921 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3__NH4_2SO4["(NH4)2SO4"]
    CultureMech_000921 --> ing3__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing3__NH4_2SO4 -.-> CHEBI_62946
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000921 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000921 --> ing5_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing6_Calf_brains["Calf brains"]
    CultureMech_000921 --> ing6_Calf_brains
    ing7_Beef_heart["Beef heart"]
    CultureMech_000921 --> ing7_Beef_heart
    ing8_Proteose_peptone["Proteose peptone"]
    CultureMech_000921 --> ing8_Proteose_peptone
    ing9_Dextrose["Dextrose"]
    CultureMech_000921 --> ing9_Dextrose
    CHEBI_17634["CHEBI:17634"]
    ing9_Dextrose -.-> CHEBI_17634
    ing10_Sodium_chloride["Sodium chloride"]
    CultureMech_000921 --> ing10_Sodium_chloride
    ing10_Sodium_chloride -.-> CHEBI_26710
    ing11_Disodium_phosphate["Disodium phosphate"]
    CultureMech_000921 --> ing11_Disodium_phosphate
    CHEBI_34683["CHEBI:34683"]
    ing11_Disodium_phosphate -.-> CHEBI_34683
    ing12_Yeast_extract["Yeast extract"]
    CultureMech_000921 --> ing12_Yeast_extract
    ing13_Sodium_resazurin["Sodium resazurin"]
    CultureMech_000921 --> ing13_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing13_Sodium_resazurin -.-> CHEBI_8806
    ing14_NaHCO3["NaHCO3"]
    CultureMech_000921 --> ing14_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing14_NaHCO3 -.-> CHEBI_32139
    ing15_Disodium_succinate["Disodium succinate"]
    CultureMech_000921 --> ing15_Disodium_succinate
    CHEBI_63675["CHEBI:63675"]
    ing15_Disodium_succinate -.-> CHEBI_63675
    ing16_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_000921 --> ing16_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing16_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing17_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_000921 --> ing17_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing17_Na2S_x_9_H2O -.-> CHEBI_76209
    ing18_Acetic_acid["Acetic acid"]
    CultureMech_000921 --> ing18_Acetic_acid
    CHEBI_15366["CHEBI:15366"]
    ing18_Acetic_acid -.-> CHEBI_15366
    ing19_Propionic_acid["Propionic acid"]
    CultureMech_000921 --> ing19_Propionic_acid
    CHEBI_30768["CHEBI:30768"]
    ing19_Propionic_acid -.-> CHEBI_30768
    ing20_Butyric_acid["Butyric acid"]
    CultureMech_000921 --> ing20_Butyric_acid
    CHEBI_30772["CHEBI:30772"]
    ing20_Butyric_acid -.-> CHEBI_30772
    ing21_n_Valeric_acid["n-Valeric acid"]
    CultureMech_000921 --> ing21_n_Valeric_acid
    CHEBI_17418["CHEBI:17418"]
    ing21_n_Valeric_acid -.-> CHEBI_17418
    ing22_iso_Butyric_acid["iso-Butyric acid"]
    CultureMech_000921 --> ing22_iso_Butyric_acid
    CHEBI_16135["CHEBI:16135"]
    ing22_iso_Butyric_acid -.-> CHEBI_16135
    ing23_Tryptone["Tryptone"]
    CultureMech_000921 --> ing23_Tryptone
    ing24_Yeast_extract["Yeast extract"]
    CultureMech_000921 --> ing24_Yeast_extract
    ing25_Sodium_chloride["Sodium chloride"]
    CultureMech_000921 --> ing25_Sodium_chloride
    ing25_Sodium_chloride -.-> CHEBI_26710
    ing26_iso_Valeric_acid["iso-Valeric acid"]
    CultureMech_000921 --> ing26_iso_Valeric_acid
    CHEBI_28484["CHEBI:28484"]
    ing26_iso_Valeric_acid -.-> CHEBI_28484
    ing27_Haemin["Haemin"]
    CultureMech_000921 --> ing27_Haemin
    CHEBI_50385["CHEBI:50385"]
    ing27_Haemin -.-> CHEBI_50385
    ing28_NaOH["NaOH"]
    CultureMech_000921 --> ing28_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing28_NaOH -.-> CHEBI_32145
    ing29_Biotin["Biotin"]
    CultureMech_000921 --> ing29_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing29_Biotin -.-> CHEBI_15956
    CultureMech_000921 --> more["...9 more"]

Ingredients (39)

Preparation

1. Dissolve ingredients except bicarbonate, succinate, vitamins, cysteine and sulfide, then sparge medium with 100% CO2 gas for 30 - 45 min to make it anoxic. Add the bicarbonate and equilibrate the medium with the CO2 gas to reach a pH between 6.5 and 7.0. Dispense medium under 100% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add succinate, vitamins, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. The vitamin solution should be sterilized by filtration. Prior to use adjust pH of complete medium to 6.6 - 6.8, if necessary.
2. Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated.

Target organisms

No target organisms are recorded.

Provenance