Overview
- External ontology
- mediadive.medium:1458 (source link unavailable) — Glucose minerals salts medium
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1458.pdf
Composition
flowchart LR
CultureMech_000924["glucose_minerals_salts_medium"]
ing0_NaNO3["NaNO3"]
CultureMech_000924 --> ing0_NaNO3
CHEBI_63005["CHEBI:63005"]
ing0_NaNO3 -.-> CHEBI_63005
ing1_K2HPO4["K2HPO4"]
CultureMech_000924 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000924 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_MnSO4_x_5_H2O["MnSO4 x 5 H2O"]
CultureMech_000924 --> ing3_MnSO4_x_5_H2O
CHEBI_131524["CHEBI:131524"]
ing3_MnSO4_x_5_H2O -.-> CHEBI_131524
ing4_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000924 --> ing4_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing4_FeSO4_x_7_H2O -.-> CHEBI_75836
ing5_CaCl2_x_7_H2O["CaCl2 x 7 H2O"]
CultureMech_000924 --> ing5_CaCl2_x_7_H2O
CHEBI_3312["CHEBI:3312"]
ing5_CaCl2_x_7_H2O -.-> CHEBI_3312
ing6_Glucose["Glucose"]
CultureMech_000924 --> ing6_Glucose
CHEBI_17234["CHEBI:17234"]
ing6_Glucose -.-> CHEBI_17234
ing7_Agar["Agar"]
CultureMech_000924 --> ing7_Agar
CHEBI_2509["CHEBI:2509"]
ing7_Agar -.-> CHEBI_2509
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaNO3
NO3.Na 84.994 g/mol
|
2 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
MnSO4 x 5 H2O
5H2O.Mn.O4S 241.076 g/mol
|
0.02 G_PER_L |
CHEBI:131524
manganese(II) sulfate pentahydrate
|
|
| 5 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.02 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 6 |
CaCl2 x 7 H2O
Ca.2Cl 110.984 g/mol
|
0.02 G_PER_L |
CHEBI:3312
calcium dichloride
|
|
| 7 |
Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17234
glucose
|
|
| 8 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
Preparation
- Autoclave glucose separately. Adjust to pH 7.0
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.495560Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1458 |
| 2026-03-13T06:18:59.617393Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000924 |
| 2026-03-17T06:29:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:11.146600+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Glucose minerals salts medium" to "glucose_minerals_salts_medium" for consistent matching |
| 2026-06-05T07:41:06.447493+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:59.122993+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T13:57:59.694168+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |