Overview
- External ontology
- mediadive.medium:1460 (source link unavailable) — 28% MH MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.5
- High-metal flag
- True
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1460.pdf
Composition
flowchart LR
CultureMech_000926["28_mh_medium"]
ing0_NaCl["NaCl"]
CultureMech_000926 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2["MgCl2"]
CultureMech_000926 --> ing1_MgCl2
CHEBI_6636["CHEBI:6636"]
ing1_MgCl2 -.-> CHEBI_6636
ing2_MgSO4["MgSO4"]
CultureMech_000926 --> ing2_MgSO4
CHEBI_32599["CHEBI:32599"]
ing2_MgSO4 -.-> CHEBI_32599
ing3_CaCl2["CaCl2"]
CultureMech_000926 --> ing3_CaCl2
CHEBI_3312["CHEBI:3312"]
ing3_CaCl2 -.-> CHEBI_3312
ing4_KCl["KCl"]
CultureMech_000926 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_NaHCO3["NaHCO3"]
CultureMech_000926 --> ing5_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing5_NaHCO3 -.-> CHEBI_32139
ing6_NaBr["NaBr"]
CultureMech_000926 --> ing6_NaBr
CHEBI_63004["CHEBI:63004"]
ing6_NaBr -.-> CHEBI_63004
ing7_Yeast_extract["Yeast extract"]
CultureMech_000926 --> ing7_Yeast_extract
ing8_Agar["Agar"]
CultureMech_000926 --> ing8_Agar
CHEBI_2509["CHEBI:2509"]
ing8_Agar -.-> CHEBI_2509
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
195 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgCl2
2Cl.Mg 95.211 g/mol
|
32.5 G_PER_L |
CHEBI:6636
magnesium dichloride
|
|
| 3 |
MgSO4
Mg.O4S 120.368 g/mol
|
50.8 G_PER_L |
CHEBI:32599
magnesium sulfate
|
|
| 4 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.8 G_PER_L |
CHEBI:3312
calcium dichloride
|
|
| 5 |
KCl
Cl.K 74.551 g/mol
|
5 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 6 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.16 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 7 |
NaBr
Br.Na 102.894 g/mol
|
0.6 G_PER_L |
CHEBI:63004
sodium bromide
|
|
| 8 | Yeast extract | 5 G_PER_L | ||
| 9 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
Preparation
- Final pH 7.5
- Add all the components together, except NaHCO3 which is added from a sterilized stock solution when the autoclaved medium has cooled. Agar is added at a concentration of 20 g/l for solid media.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.501946Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1460 |
| 2026-03-13T06:18:59.635306Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000926 |
| 2026-03-17T06:30:11.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:11.165710+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "28% MH MEDIUM" to "28_mh_medium" for consistent matching |
| 2026-06-05T07:40:15.716446+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.527156+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T13:57:59.694168+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |