Overview
- External ontology
- mediadive.medium:1465 (source link unavailable) — CHITINISPIRILLUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 10.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1465.pdf
Composition
flowchart LR
CultureMech_000931["chitinispirillum_medium"]
ing0_NaHCO3["NaHCO3"]
CultureMech_000931 --> ing0_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing0_NaHCO3 -.-> CHEBI_32139
ing1_Na2CO3["Na2CO3"]
CultureMech_000931 --> ing1_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing1_Na2CO3 -.-> CHEBI_29377
ing2_NaCl["NaCl"]
CultureMech_000931 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_K2HPO4["K2HPO4"]
CultureMech_000931 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_Chitin["Chitin"]
CultureMech_000931 --> ing4_Chitin
CHEBI_17029["CHEBI:17029"]
ing4_Chitin -.-> CHEBI_17029
ing5_MgSO4["MgSO4"]
CultureMech_000931 --> ing5_MgSO4
CHEBI_32599["CHEBI:32599"]
ing5_MgSO4 -.-> CHEBI_32599
ing6_Yeast_extract["Yeast extract"]
CultureMech_000931 --> ing6_Yeast_extract
ing7_NH4Cl["NH4Cl"]
CultureMech_000931 --> ing7_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing7_NH4Cl -.-> CHEBI_31206
ing8_Na2S["Na2S"]
CultureMech_000931 --> ing8_Na2S
CHEBI_76208["CHEBI:76208"]
ing8_Na2S -.-> CHEBI_76208
ing9_Thiamin["Thiamin"]
CultureMech_000931 --> ing9_Thiamin
CHEBI_18385["CHEBI:18385"]
ing9_Thiamin -.-> CHEBI_18385
ing10_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_000931 --> ing10_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing10_Calcium_pantothenate -.-> CHEBI_31345
ing11_B12["B12"]
CultureMech_000931 --> ing11_B12
ing12_Biotin["Biotin"]
CultureMech_000931 --> ing12_Biotin
CHEBI_15956["CHEBI:15956"]
ing12_Biotin -.-> CHEBI_15956
ing13_PABA["PABA"]
CultureMech_000931 --> ing13_PABA
CHEBI_30753["CHEBI:30753"]
ing13_PABA -.-> CHEBI_30753
ing14_Nicotinic_acid["Nicotinic acid"]
CultureMech_000931 --> ing14_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing14_Nicotinic_acid -.-> CHEBI_15940
ing15_Pyridoxine["Pyridoxine"]
CultureMech_000931 --> ing15_Pyridoxine
CHEBI_16709["CHEBI:16709"]
ing15_Pyridoxine -.-> CHEBI_16709
ing16_Folic_acid["Folic acid"]
CultureMech_000931 --> ing16_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing16_Folic_acid -.-> CHEBI_27470
ing17_Riboflavin["Riboflavin"]
CultureMech_000931 --> ing17_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing17_Riboflavin -.-> CHEBI_17015
ing18_EDTA["EDTA"]
CultureMech_000931 --> ing18_EDTA
CHEBI_4735["CHEBI:4735"]
ing18_EDTA -.-> CHEBI_4735
ing19_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000931 --> ing19_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing19_FeSO4_x_7_H2O -.-> CHEBI_75836
ing20_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000931 --> ing20_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing20_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing21_MnCl2["MnCl2"]
CultureMech_000931 --> ing21_MnCl2
CHEBI_63041["CHEBI:63041"]
ing21_MnCl2 -.-> CHEBI_63041
ing22_H3BO3["H3BO3"]
CultureMech_000931 --> ing22_H3BO3
CHEBI_33118["CHEBI:33118"]
ing22_H3BO3 -.-> CHEBI_33118
ing23_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000931 --> ing23_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing23_CoCl2_x_6_H2O -.-> CHEBI_53503
ing24_CuCl2["CuCl2"]
CultureMech_000931 --> ing24_CuCl2
CHEBI_49553["CHEBI:49553"]
ing24_CuCl2 -.-> CHEBI_49553
ing25_NiCl2_x_2_H2O["NiCl2 x 2 H2O"]
CultureMech_000931 --> ing25_NiCl2_x_2_H2O
CHEBI_34887["CHEBI:34887"]
ing25_NiCl2_x_2_H2O -.-> CHEBI_34887
ing26_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000931 --> ing26_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing26_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (27)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaHCO3
CHO3.Na 84.006 g/mol
|
8 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 2 |
Na2CO3
CO3.2Na 105.988 g/mol
|
22 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
6 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
Chitin
(C8H13NO5)n.H2O 221.209 g/mol
|
1.5 G_PER_L |
CHEBI:17029
chitin
|
(adjust in range 1 - 2 g/l if required) |
| 6 |
MgSO4
Mg.O4S 120.368 g/mol
|
0.12037 G_PER_L |
CHEBI:32599
magnesium sulfate
|
|
| 7 | Yeast extract | 0.02 G_PER_L | ||
| 8 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.21396 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 9 |
Na2S
2Na.S 78.047 g/mol
|
0.07906 G_PER_L |
CHEBI:76208
sodium sulfide (anhydrous)
|
|
| 10 |
Thiamin
C12H17N4OS 265.362 g/mol
|
0.05 G_PER_L |
CHEBI:18385
thiamine(1+)
|
|
| 11 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 12 | B12 | 0.005 G_PER_L | ||
| 13 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 14 |
PABA
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 15 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 16 |
Pyridoxine
C8H11NO3 169.18 g/mol
|
0.1 G_PER_L |
CHEBI:16709
pyridoxine
|
|
| 17 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 18 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 19 |
EDTA
C10H16N2O8 292.244 g/mol
|
5 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
|
| 20 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 21 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 22 |
MnCl2
Cl2Mn 125.844 g/mol
|
0.03 G_PER_L |
CHEBI:63041
manganese(II) chloride
|
|
| 23 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
|
| 24 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 25 |
CuCl2
Cl2Cu 134.452 g/mol
|
0.01 G_PER_L |
CHEBI:49553
copper(II) chloride
|
|
| 26 |
NiCl2 x 2 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 27 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.02 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Adjust pH to 10.0 with NaOH or HCl. Make anoxic under nitrogen stream and autoclave at 121°C.
- After autoclaving add sterile, anoxic solutions of:
- Sterilize by filtration and keep at 4°C. Before addition to the medium mix 1:1:1 (vol:vol:vol).
- adjust pH to 3-4 with HCl; autoclave in closed bottles, 120°C, 20 min.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.512906Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1465 |
| 2026-02-06T05:10:38.514659Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T06:18:59.669386Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000931 |
| 2026-03-17T06:30:26.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:11.203405+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CHITINISPIRILLUM MEDIUM" to "chitinispirillum_medium" for consistent matching |
| 2026-06-05T07:40:53.272440+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:54.462308+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 7 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-20T19:01:37.183202+00:00 | apply_mim_groundings.py | Adopted MIM published grounding | Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308). |