halococcus_medium

Overview

External ontology

mediadive.medium:146 (source link unavailable) — HALOCOCCUS MEDIUM

Applications

• Microbial cultivation

pH

8.4

High-metal flag

True

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium146.pdf

Composition

flowchart LR
    CultureMech_000936["halococcus_medium"]
    ing0_Skim_milk["Skim milk"]
    CultureMech_000936 --> ing0_Skim_milk
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000936 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_KNO3["KNO3"]
    CultureMech_000936 --> ing2_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing2_KNO3 -.-> CHEBI_63043
    ing3_NaCl["NaCl"]
    CultureMech_000936 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_Fe_III__citrate["Fe(III) citrate"]
    CultureMech_000936 --> ing4_Fe_III__citrate
    CHEBI_144421["CHEBI:144421"]
    ing4_Fe_III__citrate -.-> CHEBI_144421
    ing5_Neopeptone["Neopeptone"]
    CultureMech_000936 --> ing5_Neopeptone
    ing6_Glycerol["Glycerol"]
    CultureMech_000936 --> ing6_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing6_Glycerol -.-> CHEBI_17754
    ing7_Agar["Agar"]
    CultureMech_000936 --> ing7_Agar
    CHEBI_2509["CHEBI:2509"]
    ing7_Agar -.-> CHEBI_2509

Ingredients (8)

Preparation

1. Steam the three solutions separately, mix together solutions B and C and adjust pH to 8.4. Autoclave the skim milk at 112°C for 15 min, and the agar salt mixture at 121°C for 20 min. Add the hot mixture to the warm skim milk aseptically.
2. Use trace of Fe(III)citrate.

Target organisms

No target organisms are recorded.

Provenance