Overview
- External ontology
- mediadive.medium:1478 (source link unavailable) — NATRANAEROARCHAEUM MEDIUM AARC1
- Applications
-
- Microbial cultivation
- pH range
- {'min': 3.0, 'max': 4.0}
- High-metal flag
- True
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1478.pdf
Composition
flowchart LR
CultureMech_000945["natranaeroarchaeum_medium_aarc1"]
ing0_NaCl["NaCl"]
CultureMech_000945 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_K2HPO4["K2HPO4"]
CultureMech_000945 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2__NH4_2SO4["(NH4)2SO4"]
CultureMech_000945 --> ing2__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing2__NH4_2SO4 -.-> CHEBI_62946
ing3_Na2CO3["Na2CO3"]
CultureMech_000945 --> ing3_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing3_Na2CO3 -.-> CHEBI_29377
ing4_NaHCO3["NaHCO3"]
CultureMech_000945 --> ing4_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing4_NaHCO3 -.-> CHEBI_32139
ing5_Sulfur_powder["Sulfur powder"]
CultureMech_000945 --> ing5_Sulfur_powder
CHEBI_33403["CHEBI:33403"]
ing5_Sulfur_powder -.-> CHEBI_33403
ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000945 --> ing6_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
ing7_NH4Cl["NH4Cl"]
CultureMech_000945 --> ing7_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing7_NH4Cl -.-> CHEBI_31206
ing8_Sodium_formate["Sodium formate"]
CultureMech_000945 --> ing8_Sodium_formate
CHEBI_62965["CHEBI:62965"]
ing8_Sodium_formate -.-> CHEBI_62965
ing9_Sodium_acetate["Sodium acetate"]
CultureMech_000945 --> ing9_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing9_Sodium_acetate -.-> CHEBI_32954
ing10_Yeast_extract["Yeast extract"]
CultureMech_000945 --> ing10_Yeast_extract
ing11_Disodium_sulfide["Disodium sulfide"]
CultureMech_000945 --> ing11_Disodium_sulfide
CHEBI_76208["CHEBI:76208"]
ing11_Disodium_sulfide -.-> CHEBI_76208
ing12_EDTA["EDTA"]
CultureMech_000945 --> ing12_EDTA
CHEBI_4735["CHEBI:4735"]
ing12_EDTA -.-> CHEBI_4735
ing13_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000945 --> ing13_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing13_FeSO4_x_7_H2O -.-> CHEBI_75836
ing14_ZnSO4["ZnSO4"]
CultureMech_000945 --> ing14_ZnSO4
CHEBI_35176["CHEBI:35176"]
ing14_ZnSO4 -.-> CHEBI_35176
ing15_MnCl2["MnCl2"]
CultureMech_000945 --> ing15_MnCl2
CHEBI_63041["CHEBI:63041"]
ing15_MnCl2 -.-> CHEBI_63041
ing16_H3BO3["H3BO3"]
CultureMech_000945 --> ing16_H3BO3
CHEBI_33118["CHEBI:33118"]
ing16_H3BO3 -.-> CHEBI_33118
ing17_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000945 --> ing17_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing17_CoCl2_x_6_H2O -.-> CHEBI_53503
ing18_CuCl2_x_5_H2O["CuCl2 x 5 H2O"]
CultureMech_000945 --> ing18_CuCl2_x_5_H2O
CHEBI_91245["CHEBI:91245"]
ing18_CuCl2_x_5_H2O -.-> CHEBI_91245
ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_000945 --> ing19_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000945 --> ing20_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing21_Thiamine["Thiamine"]
CultureMech_000945 --> ing21_Thiamine
CHEBI_18385["CHEBI:18385"]
ing21_Thiamine -.-> CHEBI_18385
ing22_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_000945 --> ing22_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing22_Calcium_pantothenate -.-> CHEBI_31345
ing23_Biotin["Biotin"]
CultureMech_000945 --> ing23_Biotin
CHEBI_15956["CHEBI:15956"]
ing23_Biotin -.-> CHEBI_15956
ing24_PABA["PABA"]
CultureMech_000945 --> ing24_PABA
CHEBI_30753["CHEBI:30753"]
ing24_PABA -.-> CHEBI_30753
ing25_Nicotinic_acid["Nicotinic acid"]
CultureMech_000945 --> ing25_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing25_Nicotinic_acid -.-> CHEBI_15940
ing26_Pyridoxine["Pyridoxine"]
CultureMech_000945 --> ing26_Pyridoxine
CHEBI_16709["CHEBI:16709"]
ing26_Pyridoxine -.-> CHEBI_16709
ing27_NaOH["NaOH"]
CultureMech_000945 --> ing27_NaOH
CHEBI_32145["CHEBI:32145"]
ing27_NaOH -.-> CHEBI_32145
ing28_Folic_acid["Folic acid"]
CultureMech_000945 --> ing28_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing28_Folic_acid -.-> CHEBI_27470
ing29_Riboflavin["Riboflavin"]
CultureMech_000945 --> ing29_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing29_Riboflavin -.-> CHEBI_17015
CultureMech_000945 --> more["...1 more"]
Ingredients (31)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
72 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.125 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 4 |
Na2CO3
CO3.2Na 105.988 g/mol
|
142.5 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 5 |
NaHCO3
CHO3.Na 84.006 g/mol
|
22.605009 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
[Merged 2 duplicates: 22.5, 0.105009] |
| 6 | Sulfur powder | 2 G_PER_L |
CHEBI:33403
elemental sulfur
|
|
| 7 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.243964 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 8 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.16047 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 9 |
Sodium formate
CHO2.Na 68.007 g/mol
|
3.40035 G_PER_L |
CHEBI:62965
sodium formate
|
|
| 10 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
0.16406 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 11 | Yeast extract | 0.02 G_PER_L | ||
| 12 |
Disodium sulfide
2Na.S 78.047 g/mol
|
1 MILLIMOLAR |
CHEBI:76208
sodium sulfide (anhydrous)
|
|
| 13 |
EDTA
C10H16N2O8 292.244 g/mol
|
5 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
|
| 14 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 15 |
ZnSO4
O4S.Zn 161.453 g/mol
|
0.1 G_PER_L |
CHEBI:35176
zinc sulfate
|
|
| 16 |
MnCl2
Cl2Mn 125.844 g/mol
|
0.03 G_PER_L |
CHEBI:63041
manganese(II) chloride
|
|
| 17 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
|
| 18 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 19 |
CuCl2 x 5 H2O
Cl2Cu.5H2O 224.527 g/mol
|
0.01 G_PER_L |
CHEBI:91245
copper(II) chloride pentahydrate
|
|
| 20 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 21 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.02 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 22 |
Thiamine
C12H17N4OS 265.362 g/mol
|
0.05 G_PER_L |
CHEBI:18385
thiamine(1+)
|
|
| 23 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 24 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 25 |
PABA
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 26 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 27 |
Pyridoxine
C8H11NO3 169.18 g/mol
|
0.1 G_PER_L |
CHEBI:16709
pyridoxine
|
|
| 28 |
NaOH
HO.Na 39.997 g/mol
|
100 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 29 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 30 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 31 | B12 | 0.005 G_PER_L |
Preparation
- Prepare the liquid medium. Dispense the medium into Hungate tubes or serum bottles under gassing with N2. Steam medium for 3h on each of 3 successive days. Before inoculation add from sterile stock solutions:
- Adjust pH to 3.0-4.0 with HCl.
- Autoclave at 121 °C for 15 min.
- Note: autoclave in closed bottles
- Sterilize by filtration.
- Mix the four solutions in the ratio 1:1:1:1.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-26T17:53:14.552386Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1478 |
| 2026-02-06T05:10:28.257524Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T03:09:02.850323Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:18:59.793395Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000945 |
| 2026-03-17T06:30:08.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:11.341005+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NATRANAEROARCHAEUM MEDIUM AARC1" to "natranaeroarchaeum_medium_aarc1" for consistent matching |
| 2026-05-20T06:02:00.685009+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '3-4' -> {'min': 3.0, 'max': 4.0} |
| 2026-06-05T07:41:39.197267+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:09.376656+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 7 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |