desulfovectis_medium

CultureMech:000958 bacterial COMPLEX LIQUID
Original name: DESULFOVECTIS MEDIUM

Overview

External ontology
mediadive.medium:1490 (source link unavailable) — DESULFOVECTIS MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 6.8, 'max': 7.0}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1490.pdf

Composition

flowchart LR
    CultureMech_000958["desulfovectis_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_000958 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_NH4Cl["NH4Cl"]
    CultureMech_000958 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_K2HPO4["K2HPO4"]
    CultureMech_000958 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_KH2PO4["KH2PO4"]
    CultureMech_000958 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_KCl["KCl"]
    CultureMech_000958 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_Na2SO4["Na2SO4"]
    CultureMech_000958 --> ing5_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing5_Na2SO4 -.-> CHEBI_32149
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000958 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_000958 --> ing7_Yeast_extract
    ing8_Sodium_resazurin["Sodium resazurin"]
    CultureMech_000958 --> ing8_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing8_Sodium_resazurin -.-> CHEBI_8806
    ing9_Na2CO3["Na2CO3"]
    CultureMech_000958 --> ing9_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing9_Na2CO3 -.-> CHEBI_29377
    ing10_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_000958 --> ing10_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing10_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing11_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000958 --> ing11_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing11_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing12_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_000958 --> ing12_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing12_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing13_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_000958 --> ing13_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing13_Na2S_x_9_H2O -.-> CHEBI_76209
    ing14_HCl["HCl"]
    CultureMech_000958 --> ing14_HCl
    CHEBI_17883["CHEBI:17883"]
    ing14_HCl -.-> CHEBI_17883
    ing15_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_000958 --> ing15_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing15_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing16_ZnCl2["ZnCl2"]
    CultureMech_000958 --> ing16_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing16_ZnCl2 -.-> CHEBI_49976
    ing17_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000958 --> ing17_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing17_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing18_H3BO3["H3BO3"]
    CultureMech_000958 --> ing18_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing18_H3BO3 -.-> CHEBI_33118
    ing19_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000958 --> ing19_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing19_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing20_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000958 --> ing20_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing20_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing21_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000958 --> ing21_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing21_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000958 --> ing22_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (23)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
19.98 G_PER_L CHEBI:26710
sodium chloride
2 NH4Cl
Cl.H4N 53.492 g/mol
0.999001 G_PER_L CHEBI:31206
ammonium chloride
3 K2HPO4
HO4P.2K 174.174 g/mol
0.2997 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
4 KH2PO4
H2O4P.K 136.084 g/mol
0.2997 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
5 KCl
Cl.K 74.551 g/mol
0.0999001 G_PER_L CHEBI:32588
potassium chloride
6 Na2SO4
2Na.O4S 142.043 g/mol
1.998 G_PER_L CHEBI:32149
sodium sulfate
7 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.0999001 G_PER_L CHEBI:86158
calcium chloride dihydrate
8 Yeast extract 1.998 G_PER_L
9 Sodium resazurin
C12H7NO4 229.191 g/mol
0.0004995 G_PER_L CHEBI:8806
Resazurin
10 Na2CO3
CO3.2Na 105.988 g/mol
1.4985 G_PER_L CHEBI:29377
sodium carbonate
11 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
2.997 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
12 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
2.997 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
13 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.499501 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
14 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.499501 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
15 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
16 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
17 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
18 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
19 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
20 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
21 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
22 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
23 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Dissolve ingredients (except carbonate, thiosulfate, magnesium chloride, cysteine and sulfide), then sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. Add magnesium chloride, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of the complete medium to 6.8 - 7.0, if necessary.
  2. After inoculation pressurize cultivation vials to 1 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture.
  3. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.591399Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1490
2026-03-13T06:18:59.912619Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000958
2026-03-17T06:30:54.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:11.477488+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DESULFOVECTIS MEDIUM" to "desulfovectis_medium" for consistent matching
2026-05-20T06:01:14.701230+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '6.8-7' -> {'min': 6.8, 'max': 7.0}
2026-06-05T07:40:57.650969+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:55.976915+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.