half_strength_r2a

CultureMech:000960 bacterial COMPLEX SOLID_AGAR
Original name: HALF STRENGTH R2A

Overview

External ontology
mediadive.medium:1492 (source link unavailable) — HALF STRENGTH R2A
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1492.pdf

Composition

flowchart LR
    CultureMech_000960["half_strength_r2a"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_000960 --> ing0_Yeast_extract
    ing1_Proteose_peptone["Proteose peptone"]
    CultureMech_000960 --> ing1_Proteose_peptone
    ing2_Casamino_acids["Casamino acids"]
    CultureMech_000960 --> ing2_Casamino_acids
    ing3_Glucose["Glucose"]
    CultureMech_000960 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_Starch["Starch"]
    CultureMech_000960 --> ing4_Starch
    CHEBI_28017["CHEBI:28017"]
    ing4_Starch -.-> CHEBI_28017
    ing5_Na_pyruvate["Na-pyruvate"]
    CultureMech_000960 --> ing5_Na_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing5_Na_pyruvate -.-> CHEBI_50144
    ing6_K2HPO4["K2HPO4"]
    CultureMech_000960 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000960 --> ing7_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing8_Agar["Agar"]
    CultureMech_000960 --> ing8_Agar
    CHEBI_2509["CHEBI:2509"]
    ing8_Agar -.-> CHEBI_2509

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Yeast extract 0.25 G_PER_L
2 Proteose peptone 0.25 G_PER_L
3 Casamino acids 0.25 G_PER_L
4 Glucose
C6H12O6 180.156 g/mol
0.25 G_PER_L CHEBI:17234
glucose
5 Starch 0.25 G_PER_L CHEBI:28017
starch
6 Na-pyruvate
C3H3O3.Na 110.044 g/mol
0.25 G_PER_L CHEBI:50144
sodium pyruvate
7 K2HPO4
HO4P.2K 174.174 g/mol
0.15 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
8 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.025 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
9 Agar 15 G_PER_L CHEBI:2509
agar

Preparation

  1. Adjust pH to 7.2 with K2HPO4 or KH2PO4.
  2. Add agar after adjusting the pH, then boil to dissolve the agar
  3. Autoclave at 121 °C for 15 min.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.598841Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1492
2026-01-28T19:48:37.562114Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:18:59.931862Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000960
2026-03-17T06:30:45.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:11.498606+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HALF STRENGTH R2A" to "half_strength_r2a" for consistent matching
2026-06-05T07:41:07.183609+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:39.454263+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:46:59.323469+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-29T07:04:23.262908+00:00 medium-type-axis-migration-v1.0 Backfilled multi-axis media type Derived composition_type=UNDEFINED from deprecated medium_type=COMPLEX.
2026-10-07T13:57:59.694168+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.