omiz_pat_medium_modified

Overview

External ontology

mediadive.medium:1494 (source link unavailable) — . OMIZ-PAT Medium (modified)

Applications

• Microbial cultivation

pH

6.9

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1494.pdf Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_000962["omiz_pat_medium_modified"]
    ing0_Rabbit_serum["Rabbit serum"]
    CultureMech_000962 --> ing0_Rabbit_serum
    ing1_L_Alanine["L-Alanine"]
    CultureMech_000962 --> ing1_L_Alanine
    CHEBI_16977["CHEBI:16977"]
    ing1_L_Alanine -.-> CHEBI_16977
    ing2_L_Arginine["L-Arginine"]
    CultureMech_000962 --> ing2_L_Arginine
    CHEBI_16467["CHEBI:16467"]
    ing2_L_Arginine -.-> CHEBI_16467
    ing3_L_Asparagine["L-Asparagine"]
    CultureMech_000962 --> ing3_L_Asparagine
    CHEBI_17196["CHEBI:17196"]
    ing3_L_Asparagine -.-> CHEBI_17196
    ing4_L_Aspartic_acid["L-Aspartic acid"]
    CultureMech_000962 --> ing4_L_Aspartic_acid
    CHEBI_17053["CHEBI:17053"]
    ing4_L_Aspartic_acid -.-> CHEBI_17053
    ing5_L_Cysteine_HCl["L-Cysteine HCl"]
    CultureMech_000962 --> ing5_L_Cysteine_HCl
    CHEBI_91247["CHEBI:91247"]
    ing5_L_Cysteine_HCl -.-> CHEBI_91247
    ing6_L_Glutamine["L-Glutamine"]
    CultureMech_000962 --> ing6_L_Glutamine
    CHEBI_18050["CHEBI:18050"]
    ing6_L_Glutamine -.-> CHEBI_18050
    ing7_L_Glutamic_acid["L-Glutamic acid"]
    CultureMech_000962 --> ing7_L_Glutamic_acid
    CHEBI_16015["CHEBI:16015"]
    ing7_L_Glutamic_acid -.-> CHEBI_16015
    ing8_Glycine["Glycine"]
    CultureMech_000962 --> ing8_Glycine
    CHEBI_15428["CHEBI:15428"]
    ing8_Glycine -.-> CHEBI_15428
    ing9_L_Histidine["L-Histidine"]
    CultureMech_000962 --> ing9_L_Histidine
    CHEBI_15971["CHEBI:15971"]
    ing9_L_Histidine -.-> CHEBI_15971
    ing10_L_Isoleucine["L-Isoleucine"]
    CultureMech_000962 --> ing10_L_Isoleucine
    CHEBI_17191["CHEBI:17191"]
    ing10_L_Isoleucine -.-> CHEBI_17191
    ing11_L_Leucine["L-Leucine"]
    CultureMech_000962 --> ing11_L_Leucine
    CHEBI_15603["CHEBI:15603"]
    ing11_L_Leucine -.-> CHEBI_15603
    ing12_L_Lysine_HCl["L-Lysine HCl"]
    CultureMech_000962 --> ing12_L_Lysine_HCl
    CHEBI_53633["CHEBI:53633"]
    ing12_L_Lysine_HCl -.-> CHEBI_53633
    ing13_L_Methionine["L-Methionine"]
    CultureMech_000962 --> ing13_L_Methionine
    CHEBI_16643["CHEBI:16643"]
    ing13_L_Methionine -.-> CHEBI_16643
    ing14_L_Ornithine_HCl["L-Ornithine HCl"]
    CultureMech_000962 --> ing14_L_Ornithine_HCl
    ing15_L_Phenylalanine["L-Phenylalanine"]
    CultureMech_000962 --> ing15_L_Phenylalanine
    CHEBI_17295["CHEBI:17295"]
    ing15_L_Phenylalanine -.-> CHEBI_17295
    ing16_L_Proline["L-Proline"]
    CultureMech_000962 --> ing16_L_Proline
    CHEBI_17203["CHEBI:17203"]
    ing16_L_Proline -.-> CHEBI_17203
    ing17_L_Serine["L-Serine"]
    CultureMech_000962 --> ing17_L_Serine
    CHEBI_17115["CHEBI:17115"]
    ing17_L_Serine -.-> CHEBI_17115
    ing18_L_Threonine["L-Threonine"]
    CultureMech_000962 --> ing18_L_Threonine
    CHEBI_16857["CHEBI:16857"]
    ing18_L_Threonine -.-> CHEBI_16857
    ing19_L_Tryptophan["L-Tryptophan"]
    CultureMech_000962 --> ing19_L_Tryptophan
    CHEBI_16828["CHEBI:16828"]
    ing19_L_Tryptophan -.-> CHEBI_16828
    ing20_L_Tyrosine["L-Tyrosine"]
    CultureMech_000962 --> ing20_L_Tyrosine
    CHEBI_17895["CHEBI:17895"]
    ing20_L_Tyrosine -.-> CHEBI_17895
    ing21_L_Valine["L-Valine"]
    CultureMech_000962 --> ing21_L_Valine
    CHEBI_16414["CHEBI:16414"]
    ing21_L_Valine -.-> CHEBI_16414
    ing22_KCl["KCl"]
    CultureMech_000962 --> ing22_KCl
    CHEBI_32588["CHEBI:32588"]
    ing22_KCl -.-> CHEBI_32588
    ing23_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000962 --> ing23_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing23_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing24_NaH2PO4_x_H2O["NaH2PO4 x H2O"]
    CultureMech_000962 --> ing24_NaH2PO4_x_H2O
    CHEBI_37585["CHEBI:37585"]
    ing24_NaH2PO4_x_H2O -.-> CHEBI_37585
    ing25_NH4Cl["NH4Cl"]
    CultureMech_000962 --> ing25_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing25_NH4Cl -.-> CHEBI_31206
    ing26_Citric_acid["Citric acid"]
    CultureMech_000962 --> ing26_Citric_acid
    CHEBI_30769["CHEBI:30769"]
    ing26_Citric_acid -.-> CHEBI_30769
    ing27_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
    CultureMech_000962 --> ing27_Calcium_D_____pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing27_Calcium_D_____pantothenate -.-> CHEBI_31345
    ing28_Choline_chloride["Choline chloride"]
    CultureMech_000962 --> ing28_Choline_chloride
    CHEBI_133341["CHEBI:133341"]
    ing28_Choline_chloride -.-> CHEBI_133341
    ing29_Myo_inositol["Myo-inositol"]
    CultureMech_000962 --> ing29_Myo_inositol
    CHEBI_17268["CHEBI:17268"]
    ing29_Myo_inositol -.-> CHEBI_17268
    CultureMech_000962 --> more["...68 more"]

Ingredients (98)

Preparation

1. Add 10 ml rabbit serum, flush the medium with N2CO2, adjust the pH to 6.9 and filter-sterilize the completed medium into tubes.
2. This recipe describes the preparation of 200 ml medium, which can be used for about 4 weeks when kept at 4°C.
3. Prepare Solutions A, flush with nitrogen to make them anoxic, filter-sterilize and store at -20°C
4. Prepare Solutions B, flush with nitrogen to make them anoxic, filter-sterilize and store at -20°C
5. Prepare Solutions C, flush with nitrogen to make them anoxic, filter-sterilize and store at -20°C
6. Prepare Solutions D, flush with nitrogen to make them anoxic, filter-sterilize and store at -20°C
7. Prepare Solutions E, flush with nitrogen to make them anoxic, filter-sterilize and store at -20°C
8. Prepare Solution F, flush with nitrogen to make it anoxic, filter-sterilize and store at 4°C
9. Prepare Solutions G, flush with nitrogen to make them anoxic, filter-sterilize and store at -20°C
10. Prepare Solutions H, flush each with nitrogen to make them anoxic, filter-sterilize and store at -20°C
11. Prepare Solution I and store at -20°C
12. D,L-alpha-lipoic acid (1 mg/ml); in 2-mercaptoethanol-ethanol (1:10); store at -20°C
13. Hemin (0.2 mM) in NaOH (10 mM)
14. Homogenize 42 g fresh baker yeast in 140 ml distilled water, incubate for 24h at 56°C and centrifuge to harvest the supernatant. Adjust pH to 7 with NaOH, make anoxic, filter-sterilize and store at -80°C
15. Prepare Trace elements I, flush with nitrogen to make them anoxic, filter-sterilize and store at 4°C
16. Prepare Trace elements II, flush with nitrogen to make them anoxic, filter-sterilize and store at 4°C
17. Prepare Trace elements III, flush with nitrogen to make them anoxic, filter-sterilize and store at 4°C
18. FeSO4 x 7H2O (10 mM) in HCl (10 mM)
19. Prepare Supplements 1, flush with nitrogen to make them anoxic, filter-sterilize and store at -20°C
20. Ascorbic acid (10%; w/v); in distilled water, neutralize with NaOH
21. Prepare Supplements 2, flush with nitrogen to make them anoxic, filter-sterilize and store at -20°C
22. Glutathion (0.5 M); in distilled water, neutralize with NaOH
23. Prepare Supplements 3, flush with nitrogen to make them anoxic, filter-sterilize and store at -20°C
24. Cholesterol (1 mg/ml); in Ethanol (100%); store at -20°C
25. NaHCO3 (10%; w/v) in distilled water, store at 4°C

Target organisms

No target organisms are recorded.

Provenance