aquaspirillum_medium_ii

CultureMech:000963 bacterial COMPLEX SOLID_AGAR
Original name: AQUASPIRILLUM MEDIUM II

Overview

External ontology
mediadive.medium:1495 (source link unavailable) — AQUASPIRILLUM MEDIUM II
Applications
  • Microbial cultivation
pH range
{'min': 7.4, 'max': 7.6}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1495.pdf

Composition

flowchart LR
    CultureMech_000963["aquaspirillum_medium_ii"]
    ing0_Peptone["Peptone"]
    CultureMech_000963 --> ing0_Peptone
    ing1_Succinic_acid["Succinic acid"]
    CultureMech_000963 --> ing1_Succinic_acid
    CHEBI_15741["CHEBI:15741"]
    ing1_Succinic_acid -.-> CHEBI_15741
    ing2__NH4_2SO4["(NH4)2SO4"]
    CultureMech_000963 --> ing2__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing2__NH4_2SO4 -.-> CHEBI_62946
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000963 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000963 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_Agar["Agar"]
    CultureMech_000963 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509
    ing6_EDTA["EDTA"]
    CultureMech_000963 --> ing6_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing6_EDTA -.-> CHEBI_4735
    ing7_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000963 --> ing7_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing7_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing8_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000963 --> ing8_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing8_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing9_H3BO3["H3BO3"]
    CultureMech_000963 --> ing9_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing9_H3BO3 -.-> CHEBI_33118
    ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000963 --> ing10_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing11_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000963 --> ing11_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing11_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing12_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000963 --> ing12_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing12_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing13_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000963 --> ing13_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing13_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing14_Folic_acid["Folic acid"]
    CultureMech_000963 --> ing14_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing14_Folic_acid -.-> CHEBI_27470
    ing15_Thiamine_HCl["Thiamine HCl"]
    CultureMech_000963 --> ing15_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing15_Thiamine_HCl -.-> CHEBI_49105
    ing16_Nicotinic_acid["Nicotinic acid"]
    CultureMech_000963 --> ing16_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing16_Nicotinic_acid -.-> CHEBI_15940
    ing17_DL_Pantothenic_acid["DL-Pantothenic acid"]
    CultureMech_000963 --> ing17_DL_Pantothenic_acid
    CHEBI_7916["CHEBI:7916"]
    ing17_DL_Pantothenic_acid -.-> CHEBI_7916
    ing18_Vitamin_B12["Vitamin B12"]
    CultureMech_000963 --> ing18_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing18_Vitamin_B12 -.-> CHEBI_176843
    ing19_Para_aminobenzoic_acid["Para-aminobenzoic acid"]
    CultureMech_000963 --> ing19_Para_aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing19_Para_aminobenzoic_acid -.-> CHEBI_30753

Ingredients (20)

# Ingredient Concentration Ontology Notes
1 Peptone 2 G_PER_L
2 Succinic acid
C4H6O4 118.088 g/mol
1 G_PER_L CHEBI:15741
succinic acid
3 (NH4)2SO4
2H4N.O4S 132.141 g/mol
1 G_PER_L CHEBI:62946
ammonium sulfate
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.03 G_PER_L CHEBI:86158
calcium chloride dihydrate
6 Agar 15 G_PER_L CHEBI:2509
agar
 (for solid medium)
7 EDTA
C10H16N2O8 292.244 g/mol
5 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
8 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
9 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
10 H3BO3
H3BO3 61.833 g/mol
0.03 G_PER_L CHEBI:33118
boric acid
11 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
12 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.03 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
13 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.03 G_PER_L CHEBI:34887
nickel dichloride
14 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate
15 Folic acid
C19H19N7O6 441.404 g/mol
0.02 G_PER_L CHEBI:27470
folic acid
16 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.05 G_PER_L CHEBI:49105
thiamine hydrochloride
17 Nicotinic acid
C6H5NO2 123.111 g/mol
0.05 G_PER_L CHEBI:15940
nicotinic acid
18 DL-Pantothenic acid
C9H17NO5 219.237 g/mol
0.05 G_PER_L CHEBI:7916
pantothenic acid
19 Vitamin B12 0.001 G_PER_L CHEBI:176843
vitamin B12
20 Para-aminobenzoic acid
C7H7NO2 137.138 g/mol
0.05 G_PER_L CHEBI:30753
4-aminobenzoic acid

Preparation

  1. Adjust pH to 7.4-7.6 Autoclave and add 1.0 ml/l vitamin solution from a filter sterilised stock solution (see below). The medium may be solidified by adding 15.0 g/l agar.
  2. pH 3.0-4.0
  3. Filter sterilise.

Solutions

Vitamin solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
Biotin 0.2 G_PER_L
Pyridoxine hydrochloride 0.1 G_PER_L
Riboflavin 0.1 G_PER_L

Trace elements solution (Pfennig & Lippert, 1966)

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
FeSO4 x 6 H2O 2.2 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.617666Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1495
2026-03-13T06:18:59.995984Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000963
2026-03-17T06:30:17.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:11.567331+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "AQUASPIRILLUM MEDIUM II" to "aquaspirillum_medium_ii" for consistent matching
2026-05-20T06:01:08.888893+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7.4-7.6' -> {'min': 7.4, 'max': 7.6}
2026-06-05T07:40:49.407943+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.418150+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-07T07:05:21.635603+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 4 stock-strength component(s) out of ingredients into 2 solution(s): 3 -> 'Vitamin solution' @ 1 ml/l; 1 -> 'Trace elements solution (Pfennig & Lippert, 1966)' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).