Overview
- External ontology
- mediadive.medium:1497 (source link unavailable) — HALOARCHAEUM SALINUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 5.5
- High-metal flag
- True
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1497.pdf
Composition
flowchart LR
CultureMech_000965["haloarchaeum_salinum_medium"]
ing0_L_Glutamic_acid["L-Glutamic acid"]
CultureMech_000965 --> ing0_L_Glutamic_acid
CHEBI_16015["CHEBI:16015"]
ing0_L_Glutamic_acid -.-> CHEBI_16015
ing1_Trisodium_citrate_x_2_H2O["Trisodium citrate x 2 H2O"]
CultureMech_000965 --> ing1_Trisodium_citrate_x_2_H2O
CHEBI_53258["CHEBI:53258"]
ing1_Trisodium_citrate_x_2_H2O -.-> CHEBI_53258
ing2_KH2PO4["KH2PO4"]
CultureMech_000965 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_NH4Cl["NH4Cl"]
CultureMech_000965 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_K2SO4["K2SO4"]
CultureMech_000965 --> ing4_K2SO4
CHEBI_32036["CHEBI:32036"]
ing4_K2SO4 -.-> CHEBI_32036
ing5_MgCl2_x_7_H2O["MgCl2 x 7 H2O"]
CultureMech_000965 --> ing5_MgCl2_x_7_H2O
CHEBI_6636["CHEBI:6636"]
ing5_MgCl2_x_7_H2O -.-> CHEBI_6636
ing6_Casamino_acids["Casamino acids"]
CultureMech_000965 --> ing6_Casamino_acids
ing7_Yeast_extract["Yeast extract"]
CultureMech_000965 --> ing7_Yeast_extract
ing8_FeSO4["FeSO4"]
CultureMech_000965 --> ing8_FeSO4
CHEBI_75832["CHEBI:75832"]
ing8_FeSO4 -.-> CHEBI_75832
ing9_NaCl["NaCl"]
CultureMech_000965 --> ing9_NaCl
CHEBI_26710["CHEBI:26710"]
ing9_NaCl -.-> CHEBI_26710
ing10_Agar["Agar"]
CultureMech_000965 --> ing10_Agar
CHEBI_2509["CHEBI:2509"]
ing10_Agar -.-> CHEBI_2509
ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000965 --> ing11_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000965 --> ing12_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
ing13_ZnCl2["ZnCl2"]
CultureMech_000965 --> ing13_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing13_ZnCl2 -.-> CHEBI_49976
ing14_H3BO4["H3BO4"]
CultureMech_000965 --> ing14_H3BO4
ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000965 --> ing15_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_000965 --> ing16_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
ing17_CuCl2_x_6_H2O["CuCl2 x 6 H2O"]
CultureMech_000965 --> ing17_CuCl2_x_6_H2O
CHEBI_49553["CHEBI:49553"]
ing17_CuCl2_x_6_H2O -.-> CHEBI_49553
ing18_AlCl3["AlCl3"]
CultureMech_000965 --> ing18_AlCl3
CHEBI_30114["CHEBI:30114"]
ing18_AlCl3 -.-> CHEBI_30114
ing19_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_000965 --> ing19_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing19_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing20_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000965 --> ing20_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing20_CaCl2_x_2_H2O -.-> CHEBI_86158
ing21_BaCl2_x_2_H2O["BaCl2 x 2 H2O"]
CultureMech_000965 --> ing21_BaCl2_x_2_H2O
CHEBI_86153["CHEBI:86153"]
ing21_BaCl2_x_2_H2O -.-> CHEBI_86153
ing22_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_000965 --> ing22_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing22_Na2S2O3_x_5_H2O -.-> CHEBI_32150
Ingredients (23)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
L-Glutamic acid
C5H9NO4 147.13 g/mol
|
2.0 G_PER_L |
CHEBI:16015
L-glutamic acid
|
[Collapsed 2 identical duplicates: 2.0, 2.0] |
| 2 |
Trisodium citrate x 2 H2O
C6H5O7.3Na 258.069 g/mol
|
2.0 G_PER_L |
CHEBI:53258
sodium citrate
|
[Collapsed 2 identical duplicates: 2.0, 2.0] |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.0 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
[Collapsed 2 identical duplicates: 1.0, 1.0] |
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1.0 G_PER_L |
CHEBI:31206
ammonium chloride
|
[Collapsed 2 identical duplicates: 1.0, 1.0] |
| 5 |
K2SO4
2K.O4S 174.259 g/mol
|
5.0 G_PER_L |
CHEBI:32036
potassium sulfate
|
[Collapsed 2 identical duplicates: 5.0, 5.0] |
| 6 |
MgCl2 x 7 H2O
2Cl.Mg 95.211 g/mol
|
40.0 G_PER_L |
CHEBI:6636
magnesium dichloride
|
[Collapsed 2 identical duplicates: 40.0, 40.0] |
| 7 | Casamino acids | 4.0 G_PER_L | [Collapsed 2 identical duplicates: 4.0, 4.0] | |
| 8 | Yeast extract | 2.0 G_PER_L | [Collapsed 2 identical duplicates: 2.0, 2.0] | |
| 9 |
FeSO4
Fe.O4S 151.908 g/mol
|
0.004 G_PER_L |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
[Collapsed 2 identical duplicates: 0.004, 0.004] |
| 10 |
NaCl
Cl.Na 58.443 g/mol
|
200.0 G_PER_L |
CHEBI:26710
sodium chloride
|
[Collapsed 2 identical duplicates: 200.0, 200.0] |
| 11 | Agar | 40 G_PER_L |
CHEBI:2509
agar
|
|
| 12 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.3 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 13 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 14 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.1 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 15 | H3BO4 | 0.02 G_PER_L | ||
| 16 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 17 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.1 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 18 |
CuCl2 x 6 H2O
Cl2Cu 134.452 g/mol
|
0.01 G_PER_L |
CHEBI:49553
copper(II) chloride
|
|
| 19 |
AlCl3
AlCl3 133.341 g/mol
|
0.04 G_PER_L |
CHEBI:30114
aluminium trichloride
|
|
| 20 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.02 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 21 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 22 |
BaCl2 x 2 H2O
Ba.2Cl.2H2O 244.264 g/mol
|
0.01 G_PER_L |
CHEBI:86153
barium chloride dihydrate
|
|
| 23 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
2 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
Preparation
- Adjust to pH 5.5 with 10% KOH and sterilize by autoclaving.
- Adjust to pH 4.0.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.623068Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1497 |
| 2026-01-28T19:48:21.828971Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:09:05.875779Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 10 duplicate ingredient(s) |
| 2026-03-13T06:19:00.010423Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000965 |
| 2026-03-17T06:30:12.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:11.583065+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALOARCHAEUM SALINUM MEDIUM" to "haloarchaeum_salinum_medium" for consistent matching |
| 2026-06-05T07:41:07.499578+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:39.610222+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T07:46:59.442484+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:37:28.797169+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 10 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |