megadesulfovibrio_medium

Overview

External ontology

mediadive.medium:149 (source link unavailable) — MEGADESULFOVIBRIO MEDIUM

Applications

• Microbial cultivation

pH range

{'min': 7.0, 'max': 7.2}

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium149.pdf

Composition

flowchart LR
    CultureMech_000968["megadesulfovibrio_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_000968 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_NH4Cl["NH4Cl"]
    CultureMech_000968 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000968 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_Na2SO4["Na2SO4"]
    CultureMech_000968 --> ing3_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing3_Na2SO4 -.-> CHEBI_32149
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000968 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_H2SO4["H2SO4"]
    CultureMech_000968 --> ing5_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing5_H2SO4 -.-> CHEBI_26836
    ing6_Na_L_lactate["Na-L-lactate"]
    CultureMech_000968 --> ing6_Na_L_lactate
    CHEBI_232798["CHEBI:232798"]
    ing6_Na_L_lactate -.-> CHEBI_232798
    ing7_Sodium_resazurin["Sodium resazurin"]
    CultureMech_000968 --> ing7_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Sodium_resazurin -.-> CHEBI_8806
    ing8_Na2CO3["Na2CO3"]
    CultureMech_000968 --> ing8_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing8_Na2CO3 -.-> CHEBI_29377
    ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_000968 --> ing9_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing9_Na2S_x_9_H2O -.-> CHEBI_76209
    ing10_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000968 --> ing10_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing10_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000968 --> ing11_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing12_H3BO3["H3BO3"]
    CultureMech_000968 --> ing12_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing12_H3BO3 -.-> CHEBI_33118
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000968 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000968 --> ing14_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000968 --> ing15_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000968 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing17_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_000968 --> ing17_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing17_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing18_D_____biotin["D-(+)-biotin"]
    CultureMech_000968 --> ing18_D_____biotin
    CHEBI_15956["CHEBI:15956"]
    ing18_D_____biotin -.-> CHEBI_15956
    ing19_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_000968 --> ing19_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing19_Calcium_pantothenate -.-> CHEBI_31345

Ingredients (20)

Preparation

1. Sparge solution A with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solution B is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution C is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solution D is autoclaved separately under 100% N2 gas. To complete the medium appropriate amounts of solutions B to D are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.0 - 7.2.
2. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum.

Solutions

Target organisms

No target organisms are recorded.

Provenance