Overview
- External ontology
- mediadive.medium:14 (source link unavailable) — CLOSTRIDIUM FORMICACETICUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 8.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium14.pdf
Composition
flowchart LR
CultureMech_000969["clostridium_formicaceticum_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_000969 --> ing0_Yeast_extract
ing1_K2HPO4["K2HPO4"]
CultureMech_000969 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_000969 --> ing2_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing2_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing3_Na_thioglycolate["Na-thioglycolate"]
CultureMech_000969 --> ing3_Na_thioglycolate
CHEBI_86481["CHEBI:86481"]
ing3_Na_thioglycolate -.-> CHEBI_86481
ing4_Sodium_resazurin["Sodium resazurin"]
CultureMech_000969 --> ing4_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Sodium_resazurin -.-> CHEBI_8806
ing5_Na2CO3["Na2CO3"]
CultureMech_000969 --> ing5_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing5_Na2CO3 -.-> CHEBI_29377
ing6_D_Fructose["D-Fructose"]
CultureMech_000969 --> ing6_D_Fructose
CHEBI_15824["CHEBI:15824"]
ing6_D_Fructose -.-> CHEBI_15824
ing7_Agar["Agar"]
CultureMech_000969 --> ing7_Agar
CHEBI_2509["CHEBI:2509"]
ing7_Agar -.-> CHEBI_2509
ing8_Na2_EDTA["Na2-EDTA"]
CultureMech_000969 --> ing8_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing8_Na2_EDTA -.-> CHEBI_64734
ing9_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000969 --> ing9_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing9_FeSO4_x_7_H2O -.-> CHEBI_75836
ing10_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000969 --> ing10_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing10_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000969 --> ing11_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
ing12_H3BO3["H3BO3"]
CultureMech_000969 --> ing12_H3BO3
CHEBI_33118["CHEBI:33118"]
ing12_H3BO3 -.-> CHEBI_33118
ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000969 --> ing13_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_000969 --> ing14_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_000969 --> ing15_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000969 --> ing16_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 4.9456 G_PER_L | ||
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
9.8912 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.00098912 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 4 |
Na-thioglycolate
C2H3O2S.Na 114.101 g/mol
|
0.74184 G_PER_L |
CHEBI:86481
sodium thioglycolate
|
|
| 5 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.00049456 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 6 |
Na2CO3
CO3.2Na 105.988 g/mol
|
0.98912 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 7 |
D-Fructose
C6H12O6 180.156 g/mol
|
4.9456 G_PER_L |
CHEBI:15824
D-fructose
|
|
| 8 | Agar | 14.8368 G_PER_L |
CHEBI:2509
agar
|
Optional ingredient (for solid medium) |
| 9 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.5 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 10 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 11 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 12 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 13 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
|
| 14 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 15 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 16 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 17 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Dissolve ingredients (except carbonate and fructose) and sparge medium with 100% N2 gas for at least 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add fructose from a sterile anoxic stock solution prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Adjust pH of complete medium to 8.0.
- First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:09.981835Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 14 |
| 2026-03-13T06:19:00.049915Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000969 |
| 2026-03-17T06:30:49.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:11.626412+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CLOSTRIDIUM FORMICACETICUM MEDIUM" to "clostridium_formicaceticum_medium" for consistent matching |
| 2026-06-05T07:40:53.885647+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:54.678666+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |