acanthamoeba_medium

Overview

External ontology

mediadive.medium:1500 (source link unavailable) — ACANTHAMOEBA MEDIUM

Applications

• Microbial cultivation

pH

6.5

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1500.pdf Commercial Product: Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Supplier: Multiple suppliers (BD, Sigma, Neogen) Catalog: Multiple (22092 Sigma, 211825 BD) Source: https://en.wikipedia.org/wiki/Tryptic_soy_broth Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_000970["acanthamoeba_medium"]
    ing0_Pancreatic_digest_of_casein["Pancreatic digest of casein"]
    CultureMech_000970 --> ing0_Pancreatic_digest_of_casein
    ing1_Peptic_digest_of_soybean_meal["Peptic digest of soybean meal"]
    CultureMech_000970 --> ing1_Peptic_digest_of_soybean_meal
    ing2_Glucose["Glucose"]
    CultureMech_000970 --> ing2_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing2_Glucose -.-> CHEBI_17234
    ing3_Sodium_chloride["Sodium chloride"]
    CultureMech_000970 --> ing3_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing3_Sodium_chloride -.-> CHEBI_26710
    ing4_Dipotassium_phosphate["Dipotassium phosphate"]
    CultureMech_000970 --> ing4_Dipotassium_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing4_Dipotassium_phosphate -.-> CHEBI_131527
    ing5_Agar["Agar"]
    CultureMech_000970 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_000970 --> ing6_Yeast_extract
    ing7_Proteose_peptone["Proteose peptone"]
    CultureMech_000970 --> ing7_Proteose_peptone
    ing8_Glucose["Glucose"]
    CultureMech_000970 --> ing8_Glucose
    ing8_Glucose -.-> CHEBI_17234
    ing9_Sodium_citrate_dihydrate["Sodium citrate dihydrate"]
    CultureMech_000970 --> ing9_Sodium_citrate_dihydrate
    CHEBI_32142["CHEBI:32142"]
    ing9_Sodium_citrate_dihydrate -.-> CHEBI_32142
    ing10_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000970 --> ing10_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing10_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing11_Na2HPO4_x_7_H2O["Na2HPO4 x 7 H2O"]
    CultureMech_000970 --> ing11_Na2HPO4_x_7_H2O
    CHEBI_34683["CHEBI:34683"]
    ing11_Na2HPO4_x_7_H2O -.-> CHEBI_34683
    ing12_KH2PO4["KH2PO4"]
    CultureMech_000970 --> ing12_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing12_KH2PO4 -.-> CHEBI_63036
    ing13_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
    CultureMech_000970 --> ing13_Fe_NH4_2_SO4_2_x_6_H2O
    CHEBI_76181["CHEBI:76181"]
    ing13_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
    ing14_Sucrose["Sucrose"]
    CultureMech_000970 --> ing14_Sucrose
    CHEBI_17992["CHEBI:17992"]
    ing14_Sucrose -.-> CHEBI_17992
    ing15_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
    CultureMech_000970 --> ing15_Na2HPO4_x_2_H2O
    CHEBI_91258["CHEBI:91258"]
    ing15_Na2HPO4_x_2_H2O -.-> CHEBI_91258
    ing16_Glutamic_acid["Glutamic acid"]
    CultureMech_000970 --> ing16_Glutamic_acid
    CHEBI_18237["CHEBI:18237"]
    ing16_Glutamic_acid -.-> CHEBI_18237

Ingredients (17)

Preparation

1. Media for maintenance of infected amoeba cultures
2. OR
3. Adjust pH to 7.3, autoclave at 121°C
4. Adjust pH to 6.5, autoclave at 110°C or filter-sterilize
5. Infected amoeba cultures can be maintained e.g. in 25 cm2 flasks (e.g. Nunc flasks, VWR, 734-2081, Thermo-Scientific, 156340) at 20-30°C in an incubator (no special requirements concerning CO2 or light).
6. Infected Acanthamoeba cultures don't have to be transferred to fresh culture flasks as frequently as usually done for mammalian cell cultures. Amoeba cultures can be maintained in the same flask for several weeks or months. However, if the cultures reach a high cell density, medium should be exchanged. For that purpose amoebae are detached from the surface of the flask by knocking at the culture flask (no trypsin required, amoebae are only weakly adherent compared to most mammalian cells) and the medium (containing the majority of cells) is then completely removed. Fresh medium is added and the few remaining attached amoebae will continue to grow.
7. Alternatively, cultures can also be passaged regularly (e.g. weekly) by transfer of a small aliquot of a densely grown culture (after cell detachment, as described above) to a fresh flask containing medium.
8. If cultures contain few attached cells, but high amounts of cell debris, medium should be exchanged without prior cell detachment. Cultures usually recover fast.
9. Depending on the host cell strain, the bacteria may cause host cell lysis. To maintain such cultures, bacteria have to be transferred by adding culture supernatant (approximately 0.5 ml) to fresh culture flasks containing uninfected host cells (approximately semi-confluent culture) each 7-10 days.
10. Bacteria released from host cells can be stored in
11. at -80°C for several months.
12. Infected cell cultures (supplemented with 10% DMSO) are best stored in liquid nitrogen.

Target organisms

No target organisms are recorded.

Provenance