cultivation_medium_for_chlamydophila_pecorum

CultureMech:000973 bacterial COMPLEX LIQUID
Original name: CULTIVATION MEDIUM FOR CHLAMYDOPHILA PECORUM

Overview

External ontology
mediadive.medium:1503 (source link unavailable) — CULTIVATION MEDIUM FOR CHLAMYDOPHILA PECORUM
Applications
  • Microbial cultivation
Notes
DSMZ Medium 1503 prepares a 50.5 ml CACO-2 cell-culture medium from 45.0 ml MEM, 5.0 ml fetal bovine serum, and 0.5 ml 100x amino-acid stock, then filter-sterilizes it before Chlamydophila pecorum cultivation at 37 C plus 5% CO2.

Composition

flowchart LR
    CultureMech_000973["cultivation_medium_for_chlamydophila_pecorum"]
    ing0_MEM_Medium["MEM-Medium"]
    CultureMech_000973 --> ing0_MEM_Medium
    ing1_Fetal_bovine_serum["Fetal bovine serum"]
    CultureMech_000973 --> ing1_Fetal_bovine_serum
    ing2_Aminoacids__100_x_["Aminoacids (100 x)"]
    CultureMech_000973 --> ing2_Aminoacids__100_x_

Ingredients (3)

# Ingredient Concentration Ontology Notes
1 MEM-Medium 891.089109 ML_PER_L mediadive.compound:1377 (source link unavailable)
MEM-Medium
 DSMZ Medium 1503 lists 45.0 ml MEM-Medium in a 50.5 ml CACO-2 cell-culture medium batch.
2 Fetal bovine serum 99.009901 ML_PER_L mediadive.compound:954 (source link unavailable)
Fetal bovine serum
 DSMZ Medium 1503 lists 5.0 ml fetal bovine serum in a 50.5 ml CACO-2 cell-culture medium batch.
3 Aminoacids (100 x) 9.90099 ML_PER_L mediadive.compound:1070 (source link unavailable)
Aminoacids
 DSMZ Medium 1503 lists 0.5 ml 100x amino-acid stock in a 50.5 ml CACO-2 cell-culture medium batch.

Preparation

  1. Combine 45.0 ml MEM-Medium, 5.0 ml fetal bovine serum, and 0.5 ml 100x amino acids.
  2. Filter sterilize through a 0.2 um membrane.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.643919Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1503
2026-03-13T06:19:00.082804Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000973
2026-03-17T19:45:11.662212+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "CULTIVATION MEDIUM FOR CHLAMYDOPHILA PECORUM" to "cultivation_medium_for_chlamydophila_pecorum" for consistent matching
2026-09-09T00:00:00-07:00 repair_dsmz_host_cell_score25.py RESOLVED_DSMZ_HOST_CELL_SCORE25_GRAPH DSMZ Medium 1503 prepares a 50.5 ml CACO-2 cell-culture medium from 45.0 ml MEM, 5.0 ml fetal bovine serum, and 0.5 ml 100x amino-acid stock, then filter-sterilizes it before Chlamydophila pecorum cultivation at 37 C plus 5% CO2.