acidithiobacillus_caldus_medium

CultureMech:000981 bacterial DEFINED LIQUID
Original name: ACIDITHIOBACILLUS CALDUS MEDIUM

Overview

External ontology
mediadive.medium:150a (source link unavailable) — ACIDITHIOBACILLUS CALDUS MEDIUM
Applications
  • Microbial cultivation
pH
2.5
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium150a.pdf

Composition

flowchart LR
    CultureMech_000981["acidithiobacillus_caldus_medium"]
    ing0__NH4_2SO4["(NH4)2SO4"]
    CultureMech_000981 --> ing0__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing0__NH4_2SO4 -.-> CHEBI_62946
    ing1_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
    CultureMech_000981 --> ing1_K2HPO4_x_3_H2O
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4_x_3_H2O -.-> CHEBI_131527
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000981 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_KCl["KCl"]
    CultureMech_000981 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
    CultureMech_000981 --> ing4_Ca_NO3_2_x_4_H2O
    CHEBI_86159["CHEBI:86159"]
    ing4_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
    ing5_Sulfur["Sulfur"]
    CultureMech_000981 --> ing5_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing5_Sulfur -.-> CHEBI_26833
    ing6_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_000981 --> ing6_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing6_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing7_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000981 --> ing7_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing7_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing8_H3BO3["H3BO3"]
    CultureMech_000981 --> ing8_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing8_H3BO3 -.-> CHEBI_33118
    ing9_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_000981 --> ing9_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing9_MnSO4_x_H2O -.-> CHEBI_86364
    ing10_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000981 --> ing10_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing10_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000981 --> ing11_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing12_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000981 --> ing12_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing12_ZnSO4_x_7_H2O -.-> CHEBI_32312

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 (NH4)2SO4
2H4N.O4S 132.141 g/mol
2.9703 G_PER_L CHEBI:62946
ammonium sulfate
2 K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
0.49505 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.49505 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 KCl
Cl.K 74.551 g/mol
0.0990099 G_PER_L CHEBI:32588
potassium chloride
5 Ca(NO3)2 x 4 H2O
Ca.4H2O.2NO3 236.146 g/mol
0.019802 G_PER_L CHEBI:86159
calcium nitrate tetrahydrate
6 Sulfur
S 32.067 g/mol
4.9505 G_PER_L CHEBI:26833
sulfur atom
7 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
1.1 G_PER_L CHEBI:86254
iron trichloride hexahydrate
8 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.05 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
9 H3BO3
H3BO3 61.833 g/mol
0.2 G_PER_L CHEBI:33118
boric acid
10 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.2 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
11 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.08 G_PER_L CHEBI:75213
sodium molybdate dihydrate
12 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.06 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
13 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.09 G_PER_L CHEBI:32312
zinc sulfate heptahydrate

Preparation

  1. Dissolve ingredients except trace elements and sulfur. Adjust pH with 6 N H2SO4 to 2.5, dispense into suitable cultivation vessels and autoclave. After autoclaving add filter-sterilized trace elements solution (see below) and sterile sulfur powder. For sterilization place the sulfur in screw-capped tubes or bottles, wet with a few drops of water and heat for 3 hours to 90 - 100°C in a water bath on each of three successive days. Before use, aseptically layer the sulfur onto the surface of the autoclaved liquid basal medium.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:10.355909Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 150a
2026-03-13T06:19:00.138710Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000981
2026-03-17T06:30:15.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:11.726020+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ACIDITHIOBACILLUS CALDUS MEDIUM" to "acidithiobacillus_caldus_medium" for consistent matching
2026-06-05T07:40:47.333318+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:52.608665+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-13T00:00:00-07:00 repair_komodo_1501_acidithiobacillus_score10.py RESOLVED_KOMODO_1501_ACIDITHIOBACILLUS_PH_VARIANT Moved the DSM 18786 pH 0.8 wrapper under DSMZ Medium 150a.