Overview
- External ontology
- mediadive.medium:1521 (source link unavailable) — ARCOBACTER ANAEROPHILUS MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1521.pdf
Composition
flowchart LR
CultureMech_000992["arcobacter_anaerophilus_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_000992 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_NaCl["NaCl"]
CultureMech_000992 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000992 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000992 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_NH4Cl["NH4Cl"]
CultureMech_000992 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_Sodium_citrate["Sodium citrate"]
CultureMech_000992 --> ing5_Sodium_citrate
CHEBI_53258["CHEBI:53258"]
ing5_Sodium_citrate -.-> CHEBI_53258
ing6_Yeast_extract["Yeast extract"]
CultureMech_000992 --> ing6_Yeast_extract
ing7_Ammonium_acetate["Ammonium acetate"]
CultureMech_000992 --> ing7_Ammonium_acetate
CHEBI_62947["CHEBI:62947"]
ing7_Ammonium_acetate -.-> CHEBI_62947
ing8_Lactate["Lactate"]
CultureMech_000992 --> ing8_Lactate
CHEBI_24996["CHEBI:24996"]
ing8_Lactate -.-> CHEBI_24996
ing9_Pyruvate["Pyruvate"]
CultureMech_000992 --> ing9_Pyruvate
CHEBI_15361["CHEBI:15361"]
ing9_Pyruvate -.-> CHEBI_15361
ing10_SL10["SL10"]
CultureMech_000992 --> ing10_SL10
ing11_Resazurin["Resazurin"]
CultureMech_000992 --> ing11_Resazurin
CHEBI_8806["CHEBI:8806"]
ing11_Resazurin -.-> CHEBI_8806
ing12_NaHCO3["NaHCO3"]
CultureMech_000992 --> ing12_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing12_NaHCO3 -.-> CHEBI_32139
ing13_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_000992 --> ing13_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing13_Na2S_x_9_H2O -.-> CHEBI_76209
ing14_Vitamin_B12["Vitamin B12"]
CultureMech_000992 --> ing14_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing14_Vitamin_B12 -.-> CHEBI_176843
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
20 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.25 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.5 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 6 |
Sodium citrate
C6H5O7.3Na 258.069 g/mol
|
0.3 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 7 | Yeast extract | 0.5 G_PER_L | ||
| 8 |
Ammonium acetate
C2H3O2.H4N 77.083 g/mol
|
1 G_PER_L |
CHEBI:62947
ammonium acetate
|
|
| 9 |
Lactate
C3H5O3 89.07 g/mol
|
1 G_PER_L |
CHEBI:24996
lactate
|
|
| 10 |
Pyruvate
C3H3O3 87.054 g/mol
|
1 G_PER_L |
CHEBI:15361
pyruvate
|
|
| 11 | SL10 | 1 G_PER_L | ||
| 12 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 13 |
NaHCO3
CHO3.Na 84.006 g/mol
|
1 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 14 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
2.40182 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 15 | Vitamin B12 | 10 G_PER_L |
CHEBI:176843
vitamin B12
|
Preparation
- Add distilled water to 1 liter, boil the medium and cool under N2, distribute under N2 and autoclave. After autoclaving the following sterilized solutions are added:
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.695275Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1521 |
| 2026-03-13T06:19:00.234658Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000992 |
| 2026-03-17T06:29:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:11.831759+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ARCOBACTER ANAEROPHILUS MEDIUM" to "arcobacter_anaerophilus_medium" for consistent matching |
| 2026-06-05T07:40:49.611886+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:53.497048+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T13:57:59.694168+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |