modified_methanobacterium_medium

Overview

External ontology

mediadive.medium:1523 (source link unavailable) — MODIFIED METHANOBACTERIUM MEDIUM

Applications

• Microbial cultivation

pH range

{'min': 6.8, 'max': 7.0}

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1523.pdf Commercial Product: Brain Heart Infusion Supplier: Difco/BD Catalog: 237500 Source: https://microbenotes.com/brain-heart-infusion-bhi-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_000994["modified_methanobacterium_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_000994 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000994 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_000994 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_NH4Cl["NH4Cl"]
    CultureMech_000994 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000994 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_Calf_brains["Calf brains"]
    CultureMech_000994 --> ing5_Calf_brains
    ing6_Beef_heart["Beef heart"]
    CultureMech_000994 --> ing6_Beef_heart
    ing7_Proteose_peptone["Proteose peptone"]
    CultureMech_000994 --> ing7_Proteose_peptone
    ing8_Dextrose["Dextrose"]
    CultureMech_000994 --> ing8_Dextrose
    CHEBI_17634["CHEBI:17634"]
    ing8_Dextrose -.-> CHEBI_17634
    ing9_Sodium_chloride["Sodium chloride"]
    CultureMech_000994 --> ing9_Sodium_chloride
    ing9_Sodium_chloride -.-> CHEBI_26710
    ing10_Disodium_phosphate["Disodium phosphate"]
    CultureMech_000994 --> ing10_Disodium_phosphate
    CHEBI_34683["CHEBI:34683"]
    ing10_Disodium_phosphate -.-> CHEBI_34683
    ing11_Proteose_peptone["Proteose peptone"]
    CultureMech_000994 --> ing11_Proteose_peptone
    ing12_Yeast_extract["Yeast extract"]
    CultureMech_000994 --> ing12_Yeast_extract
    ing13_Na_acetate["Na-acetate"]
    CultureMech_000994 --> ing13_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing13_Na_acetate -.-> CHEBI_32954
    ing14_Na_formate["Na-formate"]
    CultureMech_000994 --> ing14_Na_formate
    CHEBI_62965["CHEBI:62965"]
    ing14_Na_formate -.-> CHEBI_62965
    ing15_Sodium_resazurin["Sodium resazurin"]
    CultureMech_000994 --> ing15_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing15_Sodium_resazurin -.-> CHEBI_8806
    ing16_NaHCO3["NaHCO3"]
    CultureMech_000994 --> ing16_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing16_NaHCO3 -.-> CHEBI_32139
    ing17_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_000994 --> ing17_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing17_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing18_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_000994 --> ing18_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing18_Na2S_x_9_H2O -.-> CHEBI_76209
    ing19_HCl["HCl"]
    CultureMech_000994 --> ing19_HCl
    CHEBI_17883["CHEBI:17883"]
    ing19_HCl -.-> CHEBI_17883
    ing20_ZnCl2["ZnCl2"]
    CultureMech_000994 --> ing20_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing20_ZnCl2 -.-> CHEBI_49976
    ing21_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000994 --> ing21_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing21_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing22_H3BO3["H3BO3"]
    CultureMech_000994 --> ing22_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing22_H3BO3 -.-> CHEBI_33118
    ing23_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000994 --> ing23_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing23_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing24_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000994 --> ing24_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing24_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing25_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000994 --> ing25_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing25_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing26_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000994 --> ing26_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing26_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing27_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_000994 --> ing27_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing27_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing28_D_____biotin["D-(+)-biotin"]
    CultureMech_000994 --> ing28_D_____biotin
    CHEBI_15956["CHEBI:15956"]
    ing28_D_____biotin -.-> CHEBI_15956
    ing29_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_000994 --> ing29_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing29_Calcium_pantothenate -.-> CHEBI_31345

Ingredients (30)

Preparation

1. Dissolve ingredients except bicarbonate, vitamins, cysteine, and sulfide, then sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add and dissolve bicarbonate and adjust pH to 6.5. Then dispense medium under 80% H2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. Add vitamins, cysteine, and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. The vitamin solution should be sterilized by filtration. Before use, check the complete medium's pH and adjust it to 6.8 - 7.0, if necessary.
2. After growth has started and the culture is becoming turbid add sterile 80% H2 and 20% CO2 gas mixture to 0.5 - 1 bar overpressure.
3. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Solutions

Target organisms

No target organisms are recorded.

Provenance