kiritimatiella_tyg_medium

Overview

External ontology

mediadive.medium:1526b (source link unavailable) — KIRITIMATIELLA (TYG) MEDIUM

Applications

• Microbial cultivation

pH range

{'min': 7.3, 'max': 7.5}

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1526b.pdf

Composition

flowchart LR
    CultureMech_000999["kiritimatiella_tyg_medium"]
    ing0_Sea_Salt["Sea Salt"]
    CultureMech_000999 --> ing0_Sea_Salt
    ing1_Trypticase_peptone["Trypticase peptone"]
    CultureMech_000999 --> ing1_Trypticase_peptone
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_000999 --> ing2_Yeast_extract
    ing3_Glucose["Glucose"]
    CultureMech_000999 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_Sodium_resazurin["Sodium resazurin"]
    CultureMech_000999 --> ing4_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing4_Sodium_resazurin -.-> CHEBI_8806
    ing5_Na2CO3["Na2CO3"]
    CultureMech_000999 --> ing5_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing5_Na2CO3 -.-> CHEBI_29377
    ing6_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_000999 --> ing6_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing6_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing7_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_000999 --> ing7_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing7_Na2S_x_9_H2O -.-> CHEBI_76209
    ing8_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000999 --> ing8_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing8_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing9_NaCl["NaCl"]
    CultureMech_000999 --> ing9_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing9_NaCl -.-> CHEBI_26710
    ing10_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000999 --> ing10_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing10_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing11_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000999 --> ing11_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing11_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing12_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_000999 --> ing12_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing12_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing13_H3BO3["H3BO3"]
    CultureMech_000999 --> ing13_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing13_H3BO3 -.-> CHEBI_33118
    ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000999 --> ing14_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing15__NH4_2Ni_SO4_2_x_6_H2O["(NH4)2Ni(SO4)2 x 6 H2O"]
    CultureMech_000999 --> ing15__NH4_2Ni_SO4_2_x_6_H2O
    CHEBI_86149["CHEBI:86149"]
    ing15__NH4_2Ni_SO4_2_x_6_H2O -.-> CHEBI_86149
    ing16_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_000999 --> ing16_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing16_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing17_Na2SeO4["Na2SeO4"]
    CultureMech_000999 --> ing17_Na2SeO4
    CHEBI_77775["CHEBI:77775"]
    ing17_Na2SeO4 -.-> CHEBI_77775
    ing18_Biotin["Biotin"]
    CultureMech_000999 --> ing18_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing18_Biotin -.-> CHEBI_15956
    ing19_Folic_acid["Folic acid"]
    CultureMech_000999 --> ing19_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing19_Folic_acid -.-> CHEBI_27470
    ing20_Thiamine_HCl["Thiamine HCl"]
    CultureMech_000999 --> ing20_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing20_Thiamine_HCl -.-> CHEBI_49105
    ing21_Riboflavin["Riboflavin"]
    CultureMech_000999 --> ing21_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing21_Riboflavin -.-> CHEBI_17015
    ing22_Nicotinic_acid["Nicotinic acid"]
    CultureMech_000999 --> ing22_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing22_Nicotinic_acid -.-> CHEBI_15940
    ing23_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
    CultureMech_000999 --> ing23_Calcium_D_____pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing23_Calcium_D_____pantothenate -.-> CHEBI_31345
    ing24_Vitamin_B12["Vitamin B12"]
    CultureMech_000999 --> ing24_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing24_Vitamin_B12 -.-> CHEBI_176843
    ing25_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_000999 --> ing25_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing25_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing26__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
    CultureMech_000999 --> ing26__DL__alpha_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing26__DL__alpha_Lipoic_acid -.-> CHEBI_16494

Ingredients (27)

Preparation

1. Dissolve ingredients except carbonate, vitamins, cysteine and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add glucose, vitamins, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas (vitamins are sterilized by filtration) and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 7.3 - 7.5, if necessary.
2. First adjust pH to 1.0 with diluted H2SO4, then add and dissolve the salts.

Solutions

Target organisms

No target organisms are recorded.

Provenance