halolamina_medium_nom_3

CultureMech:001005 archaea COMPLEX SOLID_AGAR
Original name: HALOLAMINA MEDIUM (NOM-3)

Overview

External ontology
mediadive.medium:1528 (source link unavailable) — HALOLAMINA MEDIUM (NOM-3)
Applications
  • Microbial cultivation
pH
7.0
High-metal flag
True
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1528.pdf

Composition

flowchart LR
    CultureMech_001005["halolamina_medium_nom_3"]
    ing0_KCl["KCl"]
    CultureMech_001005 --> ing0_KCl
    CHEBI_32588["CHEBI:32588"]
    ing0_KCl -.-> CHEBI_32588
    ing1_K2HPO4["K2HPO4"]
    CultureMech_001005 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001005 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_NH4Cl["NH4Cl"]
    CultureMech_001005 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001005 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001005 --> ing5_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing6_NaCl["NaCl"]
    CultureMech_001005 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_001005 --> ing7_Yeast_extract
    ing8_Bacto_peptone["Bacto peptone"]
    CultureMech_001005 --> ing8_Bacto_peptone
    ing9_Sodium_formate["Sodium formate"]
    CultureMech_001005 --> ing9_Sodium_formate
    CHEBI_62965["CHEBI:62965"]
    ing9_Sodium_formate -.-> CHEBI_62965
    ing10_Sodium_lactate["Sodium lactate"]
    CultureMech_001005 --> ing10_Sodium_lactate
    CHEBI_75228["CHEBI:75228"]
    ing10_Sodium_lactate -.-> CHEBI_75228
    ing11_Sodium_acetate["Sodium acetate"]
    CultureMech_001005 --> ing11_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing11_Sodium_acetate -.-> CHEBI_32954
    ing12_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_001005 --> ing12_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing12_Sodium_pyruvate -.-> CHEBI_50144
    ing13_Agar["Agar"]
    CultureMech_001005 --> ing13_Agar
    CHEBI_2509["CHEBI:2509"]
    ing13_Agar -.-> CHEBI_2509

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 KCl
Cl.K 74.551 g/mol
5.4 G_PER_L CHEBI:32588
potassium chloride
2 K2HPO4
HO4P.2K 174.174 g/mol
0.3 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.25 G_PER_L CHEBI:86158
calcium chloride dihydrate
4 NH4Cl
Cl.H4N 53.492 g/mol
0.25 G_PER_L CHEBI:31206
ammonium chloride
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
26.8 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
23 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
7 NaCl
Cl.Na 58.443 g/mol
184 G_PER_L CHEBI:26710
sodium chloride
8 Yeast extract 1 G_PER_L
9 Bacto peptone 0.5 G_PER_L
10 Sodium formate
CHO2.Na 68.007 g/mol
0.25 G_PER_L CHEBI:62965
sodium formate
11 Sodium lactate
C3H5O3.Na 112.06 g/mol
0.25 G_PER_L CHEBI:75228
sodium lactate
12 Sodium acetate
C2H3O2.Na 82.034 g/mol
0.25 G_PER_L CHEBI:32954
sodium acetate
13 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
0.25 G_PER_L CHEBI:50144
sodium pyruvate
14 Agar 20 G_PER_L CHEBI:2509
agar

Preparation

  1. Adjust pH to 7.0

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.753571Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1528
2026-03-13T06:19:00.416345Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001005
2026-03-17T06:29:46.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:12.029831+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HALOLAMINA MEDIUM (NOM-3)" to "halolamina_medium_nom_3" for consistent matching
2026-06-05T07:41:07.836454+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.539321+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T13:57:59.694168+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.