Overview
- External ontology
- mediadive.medium:1529 (source link unavailable) — GEOGLOBUS AHANGARI MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 6.8, 'max': 7.0}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1529.pdf
Composition
flowchart LR
CultureMech_001006["geoglobus_ahangari_medium"]
ing0_Ferric_citrate["Ferric citrate"]
CultureMech_001006 --> ing0_Ferric_citrate
CHEBI_144421["CHEBI:144421"]
ing0_Ferric_citrate -.-> CHEBI_144421
ing1_NaCl["NaCl"]
CultureMech_001006 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001006 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001006 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001006 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_KCl["KCl"]
CultureMech_001006 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_KH2PO4["KH2PO4"]
CultureMech_001006 --> ing6_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing6_KH2PO4 -.-> CHEBI_63036
ing7__NH4_2SO4["(NH4)2SO4"]
CultureMech_001006 --> ing7__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing7__NH4_2SO4 -.-> CHEBI_62946
ing8_NaBr["NaBr"]
CultureMech_001006 --> ing8_NaBr
CHEBI_63004["CHEBI:63004"]
ing8_NaBr -.-> CHEBI_63004
ing9_SrCl2["SrCl2"]
CultureMech_001006 --> ing9_SrCl2
CHEBI_36383["CHEBI:36383"]
ing9_SrCl2 -.-> CHEBI_36383
ing10_NaHCO3["NaHCO3"]
CultureMech_001006 --> ing10_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing10_NaHCO3 -.-> CHEBI_32139
ing11_Yeast_extract["Yeast extract"]
CultureMech_001006 --> ing11_Yeast_extract
ing12_Na_pyruvate["Na-pyruvate"]
CultureMech_001006 --> ing12_Na_pyruvate
CHEBI_50144["CHEBI:50144"]
ing12_Na_pyruvate -.-> CHEBI_50144
ing13_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_001006 --> ing13_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing13_FeCl2_x_4_H2O -.-> CHEBI_86249
ing14_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_001006 --> ing14_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing14_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing15_HCl["HCl"]
CultureMech_001006 --> ing15_HCl
CHEBI_17883["CHEBI:17883"]
ing15_HCl -.-> CHEBI_17883
ing16_ZnCl2["ZnCl2"]
CultureMech_001006 --> ing16_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing16_ZnCl2 -.-> CHEBI_49976
ing17_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001006 --> ing17_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing17_MnCl2_x_4_H2O -.-> CHEBI_86368
ing18_H3BO3["H3BO3"]
CultureMech_001006 --> ing18_H3BO3
CHEBI_33118["CHEBI:33118"]
ing18_H3BO3 -.-> CHEBI_33118
ing19_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001006 --> ing19_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing19_CoCl2_x_6_H2O -.-> CHEBI_53503
ing20_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001006 --> ing20_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing20_CuCl2_x_2_H2O -.-> CHEBI_86318
ing21_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001006 --> ing21_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing21_NiCl2_x_6_H2O -.-> CHEBI_34887
ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001006 --> ing22_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing23_Biotin["Biotin"]
CultureMech_001006 --> ing23_Biotin
CHEBI_15956["CHEBI:15956"]
ing23_Biotin -.-> CHEBI_15956
ing24_Folic_acid["Folic acid"]
CultureMech_001006 --> ing24_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing24_Folic_acid -.-> CHEBI_27470
ing25_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001006 --> ing25_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing25_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing26_Thiamine_HCl["Thiamine HCl"]
CultureMech_001006 --> ing26_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing26_Thiamine_HCl -.-> CHEBI_49105
ing27_Riboflavin["Riboflavin"]
CultureMech_001006 --> ing27_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing27_Riboflavin -.-> CHEBI_17015
ing28_Nicotinic_acid["Nicotinic acid"]
CultureMech_001006 --> ing28_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing28_Nicotinic_acid -.-> CHEBI_15940
ing29_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_001006 --> ing29_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing29_Calcium_D_____pantothenate -.-> CHEBI_31345
CultureMech_001006 --> more["...3 more"]
Ingredients (33)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Ferric citrate
C6H5FeO7 244.944 g/mol
|
11.1776 G_PER_L |
CHEBI:144421
iron(III) citrate
|
|
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
18.9621 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
8.98204 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.149701 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.299401 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.0998004 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 7 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.598802 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 8 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.0998004 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 9 |
NaBr
Br.Na 102.894 g/mol
|
0.0499002 G_PER_L |
CHEBI:63004
sodium bromide
|
|
| 10 |
SrCl2
2Cl.Sr 158.526 g/mol
|
0.0199601 G_PER_L |
CHEBI:36383
strontium dichloride
|
|
| 11 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2.49501 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 12 | Yeast extract | 0.0998004 G_PER_L | ||
| 13 |
Na-pyruvate
C3H3O3.Na 110.044 g/mol
|
0.998004 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 14 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.699601 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
[Merged 2 duplicates: 0.199601, 1.5] |
| 15 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.0998004 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 16 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 17 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 18 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 19 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 20 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 21 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 22 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 23 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 24 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 25 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 26 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 27 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 28 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 29 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 30 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 31 | Vitamin B12 | 0.001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 32 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 33 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- First, dissolve ferric citrate in water by heating, adjust to pH 5.8, then cool to room temperature. Add other compounds (except bicarbonate, yeast extract, pyruvate, vitamins, cysteine, and ferrous chloride) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Then add cysteine and ferrous chloride and adjust pH to 6.8 - 7.0 with bicarbonate. Dispense medium under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Before inoculation add yeast extract, pyruvate, and vitamins, from sterile anoxic stock solutions prepared under 100% N2 gas. Stock solutions of pyruvate and vitamins should be sterilized by filtration. Adjust the pH of the complete medium to 6.8 - 7.0.
- Note: Use 5 - 10% (v/v) inoculum. The color of the medium supernatant changes from orange-brown to yellow-greenish during growth.
- First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.755944Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1529 |
| 2026-02-06T05:10:40.408909Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T03:09:22.079791Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:00.428962Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001006 |
| 2026-03-17T06:30:30.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:12.043500+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "GEOGLOBUS AHANGARI MEDIUM" to "geoglobus_ahangari_medium" for consistent matching |
| 2026-05-20T06:01:21.010673+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '6.8-7' -> {'min': 6.8, 'max': 7.0} |
| 2026-06-05T07:41:06.212618+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:59.046941+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |