Overview
- External ontology
- mediadive.medium:152 (source link unavailable) — NICOTINIC ACID MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 8.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium152.pdf
Composition
flowchart LR
CultureMech_001007["nicotinic_acid_medium"]
ing0_Nicotinic_acid["Nicotinic acid"]
CultureMech_001007 --> ing0_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing0_Nicotinic_acid -.-> CHEBI_15940
ing1_Yeast_extract["Yeast extract"]
CultureMech_001007 --> ing1_Yeast_extract
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001007 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001007 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_KH2PO4["KH2PO4"]
CultureMech_001007 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_K2HPO4["K2HPO4"]
CultureMech_001007 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_NaCl["NaCl"]
CultureMech_001007 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_NaHCO3["NaHCO3"]
CultureMech_001007 --> ing7_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing7_NaHCO3 -.-> CHEBI_32139
ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_001007 --> ing8_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
5 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 2 | Yeast extract | 10 G_PER_L | ||
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.008 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.008 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.04 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.04 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
0.08 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 8 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.4 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 9 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Preparation
- Add nicotinic acid to the given amount of water, boil until dissolved and cool to room temperature. Add the other substances, adjust pH to 8.2 and distribute under 100% nitrogen atmosphere.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.358102Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 152 |
| 2026-03-13T06:19:00.437779Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001007 |
| 2026-03-17T06:30:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:12.053490+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NICOTINIC ACID MEDIUM" to "nicotinic_acid_medium" for consistent matching |
| 2026-06-05T07:41:39.664314+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:09.546736+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T10:55:41.454416+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |