bap_medium_for_frankia

CultureMech:001014 bacterial DEFINED LIQUID
Original name: BAP+ MEDIUM FOR FRANKIA

Overview

External ontology
mediadive.medium:1536 (source link unavailable) — BAP+ MEDIUM FOR FRANKIA
Applications
  • Microbial cultivation
pH
6.3
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1536.pdf

Composition

flowchart LR
    CultureMech_001014["bap_medium_for_frankia"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_001014 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_K2HPO4["K2HPO4"]
    CultureMech_001014 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_NH4Cl["NH4Cl"]
    CultureMech_001014 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_Na_propionate["Na-propionate"]
    CultureMech_001014 --> ing3_Na_propionate
    CHEBI_132106["CHEBI:132106"]
    ing3_Na_propionate -.-> CHEBI_132106
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001014 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001014 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_Citric_acid["Citric acid"]
    CultureMech_001014 --> ing6_Citric_acid
    CHEBI_30769["CHEBI:30769"]
    ing6_Citric_acid -.-> CHEBI_30769
    ing7_Eisencitrat["Eisencitrat"]
    CultureMech_001014 --> ing7_Eisencitrat
    CHEBI_144434["CHEBI:144434"]
    ing7_Eisencitrat -.-> CHEBI_144434
    ing8_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_001014 --> ing8_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing8_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing9_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001014 --> ing9_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing9_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing10_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001014 --> ing10_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing10_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.95 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 K2HPO4
HO4P.2K 174.174 g/mol
0.6 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
0.27 G_PER_L CHEBI:31206
ammonium chloride
4 Na-propionate
C3H5O2.Na 96.061 g/mol
0.48 G_PER_L CHEBI:132106
sodium propionate
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.025 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.01 G_PER_L CHEBI:86158
calcium chloride dihydrate
7 Citric acid
C6H8O7 192.123 g/mol
10 G_PER_L CHEBI:30769
citric acid
8 Eisencitrat
C6H5O7.Fe.H2O 262.959 g/mol
10 G_PER_L CHEBI:144434
iron(III) citrate monohydrate
9 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.01 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
10 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.8 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
11 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.25 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. The pH of the final solution is adjusted to 6.3.
  2. Note: Please autoclave the chelated iron solution before you add to the medium, so that solves everything!!!!

Solutions

Vitamine solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
Thiamine HCl 0.1 G_PER_L
Nicotinic acid 0.5 G_PER_L
Pyridoxine hydrochloride 0.5 G_PER_L

Trace element solution

Dose: 0.1 ML_PER_L

Stock composition
IngredientConcentrationNotes
H3BO3 28.6 G_PER_L
MnCl2 x 4 H2O 18.1 G_PER_L
ZnSO4 x 7 H2O 2.2 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.778433Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1536
2026-03-13T06:19:00.494245Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001014
2026-03-17T06:29:47.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:12.115555+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "BAP+ MEDIUM FOR FRANKIA" to "bap_medium_for_frankia" for consistent matching
2026-06-05T07:40:50.485224+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.788289+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-07T07:05:21.648212+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 6 stock-strength component(s) out of ingredients into 2 solution(s): 3 -> 'Vitamine solution' @ 1 ml/l; 3 -> 'Trace element solution' @ 0.1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).