Overview
- External ontology
- mediadive.medium:1542 (source link unavailable) — CY/H LIQUID MEDIUM FOR MYXOBACTERIA
- Applications
-
- Microbial cultivation
- pH
- 7.4
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1542.pdf
Composition
flowchart LR
CultureMech_001020["cy_h_liquid_medium_for_myxobacteria"]
ing0_Casitone["Casitone"]
CultureMech_001020 --> ing0_Casitone
ing1_Yeast_extract["Yeast extract"]
CultureMech_001020 --> ing1_Yeast_extract
ing2_Starch["Starch"]
CultureMech_001020 --> ing2_Starch
CHEBI_28017["CHEBI:28017"]
ing2_Starch -.-> CHEBI_28017
ing3_Soy_flour["Soy flour"]
CultureMech_001020 --> ing3_Soy_flour
ing4_D_Glucose["D-Glucose"]
CultureMech_001020 --> ing4_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing4_D_Glucose -.-> CHEBI_17634
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001020 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001020 --> ing6_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
ing7_HEPES["HEPES"]
CultureMech_001020 --> ing7_HEPES
CHEBI_46756["CHEBI:46756"]
ing7_HEPES -.-> CHEBI_46756
ing8_Fe_III__EDTA["Fe(III)-EDTA"]
CultureMech_001020 --> ing8_Fe_III__EDTA
CHEBI_30729["CHEBI:30729"]
ing8_Fe_III__EDTA -.-> CHEBI_30729
ing9_Vitamin_B12["Vitamin B12"]
CultureMech_001020 --> ing9_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing9_Vitamin_B12 -.-> CHEBI_176843
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Casitone | 1.5 G_PER_L | ||
| 2 | Yeast extract | 1.5 G_PER_L | ||
| 3 | Starch | 4 G_PER_L |
CHEBI:28017
starch
|
|
| 4 | Soy flour | 1 G_PER_L | (defatted) | |
| 5 |
D-Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 7 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 8 | HEPES | 11.9 G_PER_L |
CHEBI:46756
HEPES
|
|
| 9 |
Fe(III)-EDTA
C10H12N2O8.Fe 344.057 g/mol
|
8 G_PER_L |
CHEBI:30729
ethylenediaminetetraacetatoferrate(1-)
|
|
| 10 | Vitamin B12 | 0.5 G_PER_L |
CHEBI:176843
vitamin B12
|
Preparation
- Adjust pH of solution A to pH 7.4. Autoclave solutions A and B separately. Filter sterilize vitamin solution. After cooling, add 1 ml solution B and 1 ml solution C per liter solution A.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.794854Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1542 |
| 2026-03-13T06:19:00.539803Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001020 |
| 2026-03-17T06:30:41.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:12.166731+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CY/H LIQUID MEDIUM FOR MYXOBACTERIA" to "cy_h_liquid_medium_for_myxobacteria" for consistent matching |
| 2026-06-05T07:40:55.030031+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |