bm7_medium

CultureMech:001033 bacterial COMPLEX SOLID_AGAR
Original name: BM7 MEDIUM

Overview

External ontology
mediadive.medium:1556 (source link unavailable) — BM7 MEDIUM
Applications
  • Microbial cultivation
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1556.pdf

Composition

flowchart LR
    CultureMech_001033["bm7_medium"]
    ing0_ACES["ACES"]
    CultureMech_001033 --> ing0_ACES
    CHEBI_39061["CHEBI:39061"]
    ing0_ACES -.-> CHEBI_39061
    ing1_Na2_alpha_ketoglutarate["Na2 alpha-ketoglutarate"]
    CultureMech_001033 --> ing1_Na2_alpha_ketoglutarate
    CHEBI_16810["CHEBI:16810"]
    ing1_Na2_alpha_ketoglutarate -.-> CHEBI_16810
    ing2_KOH["KOH"]
    CultureMech_001033 --> ing2_KOH
    CHEBI_32035["CHEBI:32035"]
    ing2_KOH -.-> CHEBI_32035
    ing3_Agar["Agar"]
    CultureMech_001033 --> ing3_Agar
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar -.-> CHEBI_2509
    ing4_Fetal_bovine_serum["Fetal bovine serum"]
    CultureMech_001033 --> ing4_Fetal_bovine_serum
    ing5_TNM_FH_Medium["TNM-FH Medium"]
    CultureMech_001033 --> ing5_TNM_FH_Medium

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 ACES 10 G_PER_L CHEBI:39061
ACES
2 Na2 alpha-ketoglutarate
C5H4O5 144.082 g/mol
2 G_PER_L CHEBI:16810
2-oxoglutarate(2-)
3 KOH
HO.K 56.105 g/mol
3.75 G_PER_L CHEBI:32035
potassium hydroxide
4 Agar 15 G_PER_L CHEBI:2509
agar
 (for solid medium)
5 Fetal bovine serum 150 G_PER_L
6 TNM-FH Medium 300 G_PER_L

Preparation

  1. Cultivation of Liberibacter sp.
  2. adjust pH to 6.9, autoclave for 15 minutes and allow to cool.
  3. Add aseptically
  4. For preparation of agar plates add 15 g agar per liter of medium.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.819348Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1556
2026-03-13T06:19:00.595469Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001033
2026-03-17T06:30:55.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:12.227311+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "BM7 MEDIUM" to "bm7_medium" for consistent matching
2026-06-05T07:40:51.175418+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:34.593224+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T13:57:59.694168+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.