haha100_medium

CultureMech:001040 bacterial COMPLEX LIQUID
Original name: HAHA100-MEDIUM

Overview

External ontology
mediadive.medium:1564 (source link unavailable) — HAHA100-MEDIUM
Applications
  • Microbial cultivation
pH
7.5
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1564.pdf

Composition

flowchart LR
    CultureMech_001040["haha100_medium"]
    ing0_NaHCO3["NaHCO3"]
    CultureMech_001040 --> ing0_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing0_NaHCO3 -.-> CHEBI_32139
    ing1___D_Glucose_monohydrate["α-D-Glucose monohydrate"]
    CultureMech_001040 --> ing1___D_Glucose_monohydrate
    ing2_Cellobiose["Cellobiose"]
    CultureMech_001040 --> ing2_Cellobiose
    CHEBI_17057["CHEBI:17057"]
    ing2_Cellobiose -.-> CHEBI_17057
    ing3_Na2_EDTA["Na2-EDTA"]
    CultureMech_001040 --> ing3_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing3_Na2_EDTA -.-> CHEBI_64734
    ing4_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_001040 --> ing4_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing4_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing5_ZnCl2["ZnCl2"]
    CultureMech_001040 --> ing5_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing5_ZnCl2 -.-> CHEBI_49976
    ing6_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001040 --> ing6_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing6_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing7_H3BO3["H3BO3"]
    CultureMech_001040 --> ing7_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing7_H3BO3 -.-> CHEBI_33118
    ing8_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001040 --> ing8_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing8_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing9_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001040 --> ing9_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing9_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing10_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001040 --> ing10_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing10_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing11_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001040 --> ing11_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing11_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing12_NaCl["NaCl"]
    CultureMech_001040 --> ing12_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing12_NaCl -.-> CHEBI_26710
    ing13_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001040 --> ing13_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing13_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing14_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001040 --> ing14_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing14_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing15_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001040 --> ing15_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing15_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing16_KCl["KCl"]
    CultureMech_001040 --> ing16_KCl
    CHEBI_32588["CHEBI:32588"]
    ing16_KCl -.-> CHEBI_32588
    ing17_KBr["KBr"]
    CultureMech_001040 --> ing17_KBr
    CHEBI_32030["CHEBI:32030"]
    ing17_KBr -.-> CHEBI_32030
    ing18_NH4Cl["NH4Cl"]
    CultureMech_001040 --> ing18_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing18_NH4Cl -.-> CHEBI_31206
    ing19_KH2PO4["KH2PO4"]
    CultureMech_001040 --> ing19_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing19_KH2PO4 -.-> CHEBI_63036
    ing20_SrCl2["SrCl2"]
    CultureMech_001040 --> ing20_SrCl2
    CHEBI_36383["CHEBI:36383"]
    ing20_SrCl2 -.-> CHEBI_36383
    ing21_NaF["NaF"]
    CultureMech_001040 --> ing21_NaF
    CHEBI_28741["CHEBI:28741"]
    ing21_NaF -.-> CHEBI_28741
    ing22_Tryptone_peptone["Tryptone peptone"]
    CultureMech_001040 --> ing22_Tryptone_peptone
    ing23_Casamino_acids["Casamino acids"]
    CultureMech_001040 --> ing23_Casamino_acids
    ing24_Yeast_extract["Yeast extract"]
    CultureMech_001040 --> ing24_Yeast_extract
    ing25_NaOH["NaOH"]
    CultureMech_001040 --> ing25_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing25_NaOH -.-> CHEBI_32145
    ing26_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001040 --> ing26_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing26_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing27_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001040 --> ing27_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing27_Na2WO4_x_2_H2O -.-> CHEBI_63939

Ingredients (28)

# Ingredient Concentration Ontology Notes
1 NaHCO3
CHO3.Na 84.006 g/mol
2.17 G_PER_L CHEBI:32139
sodium hydrogencarbonate
 [Merged 2 duplicates: 0.17, 2.0]
2 α-D-Glucose monohydrate 1.4 G_PER_L [Merged 2 duplicates: 0.4, 1.0]
3 Cellobiose
C12H22O11 342.297 g/mol
1.4 G_PER_L CHEBI:17057
cellobiose
 [Merged 2 duplicates: 0.4, 1.0]
4 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
5.2 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
5 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
6 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
7 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
8 H3BO3
H3BO3 61.833 g/mol
0.082 G_PER_L CHEBI:33118
boric acid
 [Merged 2 duplicates: 0.062, 0.02]
9 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
10 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.017 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
11 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
12 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
13 NaCl
Cl.Na 58.443 g/mol
26.37 G_PER_L CHEBI:26710
sodium chloride
14 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
6.8 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
15 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
5.67 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
16 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1.47 G_PER_L CHEBI:86158
calcium chloride dihydrate
17 KCl
Cl.K 74.551 g/mol
0.72 G_PER_L CHEBI:32588
potassium chloride
18 KBr
Br.K 119.002 g/mol
0.1 G_PER_L CHEBI:32030
potassium bromide
19 NH4Cl
Cl.H4N 53.492 g/mol
0.8 MG_PER_L CHEBI:31206
ammonium chloride
20 KH2PO4
H2O4P.K 136.084 g/mol
0.02 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
21 SrCl2
2Cl.Sr 158.526 g/mol
0.02 G_PER_L CHEBI:36383
strontium dichloride
22 NaF
F.Na 41.988 g/mol
3 MG_PER_L CHEBI:28741
sodium fluoride
23 Tryptone peptone 0.1 G_PER_L
24 Casamino acids 0.1 G_PER_L
25 Yeast extract 0.1 G_PER_L
26 NaOH
HO.Na 39.997 g/mol
0.5 G_PER_L CHEBI:32145
sodium hydroxide
27 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
28 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.004 G_PER_L CHEBI:63939
sodium tungstate dihydrate

Preparation

  1. Autoclave at 121 °C for 20 min.
  2. Before inoculation add from sterile stock solutions:
  3. Adjust pH to 7.5 with 1 M HCl or 1 M NaHCO3
  4. Adjust pH to 7.5 with 1 M HCl or 1 M NaHCO3
  5. Autoclave at 121 °C for 20 min.
  6. Before inoculation add from sterile stock solutions:

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.846481Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1564
2026-01-28T19:48:30.720969Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T03:09:22.424996Z data-quality-cleanup-v1.0 Data quality fixes Merged 4 duplicate ingredient(s)
2026-03-13T06:19:00.655818Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001040
2026-03-17T06:30:30.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:12.287866+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HAHA100-MEDIUM" to "haha100_medium" for consistent matching
2026-06-05T07:41:06.967881+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:39.401948+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md.
2026-06-10T07:46:59.248868+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.