methyloprofundus_sedimenti_medium

CultureMech:001043 bacterial DEFINED LIQUID
Original name: METHYLOPROFUNDUS SEDIMENTI MEDIUM

Overview

External ontology
mediadive.medium:1567 (source link unavailable) — METHYLOPROFUNDUS SEDIMENTI MEDIUM
Applications
  • Microbial cultivation
High-metal flag
True
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1567.pdf

Composition

flowchart LR
    CultureMech_001043["methyloprofundus_sedimenti_medium"]
    ing0_KNO3["KNO3"]
    CultureMech_001043 --> ing0_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing0_KNO3 -.-> CHEBI_63043
    ing1_MgSO4_x_6_H2O["MgSO4 x 6 H2O"]
    CultureMech_001043 --> ing1_MgSO4_x_6_H2O
    CHEBI_32599["CHEBI:32599"]
    ing1_MgSO4_x_6_H2O -.-> CHEBI_32599
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001043 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
    CultureMech_001043 --> ing3_Na2HPO4_x_12_H2O
    CHEBI_34683["CHEBI:34683"]
    ing3_Na2HPO4_x_12_H2O -.-> CHEBI_34683
    ing4_KH2PO4["KH2PO4"]
    CultureMech_001043 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_001043 --> ing5_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing5_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001043 --> ing6_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing7_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001043 --> ing7_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing7_MnSO4_x_H2O -.-> CHEBI_86364
    ing8_NaCl["NaCl"]
    CultureMech_001043 --> ing8_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing8_NaCl -.-> CHEBI_26710
    ing9_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001043 --> ing9_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing9_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing10_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_001043 --> ing10_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing10_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing11_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001043 --> ing11_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing11_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing12_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001043 --> ing12_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing12_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing13_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_001043 --> ing13_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing13_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing14_H3BO3["H3BO3"]
    CultureMech_001043 --> ing14_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing14_H3BO3 -.-> CHEBI_33118
    ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001043 --> ing15_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001043 --> ing16_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing17_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001043 --> ing17_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing17_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing18_Biotin["Biotin"]
    CultureMech_001043 --> ing18_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing18_Biotin -.-> CHEBI_15956
    ing19_Folic_acid["Folic acid"]
    CultureMech_001043 --> ing19_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing19_Folic_acid -.-> CHEBI_27470
    ing20_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_001043 --> ing20_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing20_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing21_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_001043 --> ing21_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing21_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing22_Riboflavin["Riboflavin"]
    CultureMech_001043 --> ing22_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing22_Riboflavin -.-> CHEBI_17015
    ing23_Nicotinic_acid["Nicotinic acid"]
    CultureMech_001043 --> ing23_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing23_Nicotinic_acid -.-> CHEBI_15940
    ing24_D_Calcium_pantothenate["D-Calcium pantothenate"]
    CultureMech_001043 --> ing24_D_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing24_D_Calcium_pantothenate -.-> CHEBI_31345
    ing25_Vitamin_B12["Vitamin B12"]
    CultureMech_001043 --> ing25_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing25_Vitamin_B12 -.-> CHEBI_176843
    ing26_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_001043 --> ing26_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing26_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing27_Lipoic_acid["Lipoic acid"]
    CultureMech_001043 --> ing27_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing27_Lipoic_acid -.-> CHEBI_16494
    ing28_Fe_III__EDTA["Fe(III)-EDTA"]
    CultureMech_001043 --> ing28_Fe_III__EDTA
    CHEBI_30729["CHEBI:30729"]
    ing28_Fe_III__EDTA -.-> CHEBI_30729

Ingredients (29)

# Ingredient Concentration Ontology Notes
1 KNO3
K.NO3 101.102 g/mol
10 G_PER_L CHEBI:63043
potassium nitrate
2 MgSO4 x 6 H2O
Mg.O4S 120.368 g/mol
10 G_PER_L CHEBI:32599
magnesium sulfate
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
2.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 2.0, 0.1]
4 Na2HPO4 x 12 H2O
HO4P.2Na 141.958 g/mol
71.6 G_PER_L CHEBI:34683
disodium hydrogenphosphate
5 KH2PO4
H2O4P.K 136.084 g/mol
26 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
6 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
7 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
8 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.5 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
9 NaCl
Cl.Na 58.443 g/mol
301.0 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 1.0, 300.0]
10 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
11 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.18 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
12 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
13 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
14 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.02 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
15 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
16 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
17 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.03 G_PER_L CHEBI:34887
nickel dichloride
18 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.0003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
19 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
20 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
21 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
22 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.005 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
23 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
24 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
25 D-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
26 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
27 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
28 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid
29 Fe(III)-EDTA
C10H12N2O8.Fe 344.057 g/mol
3.8 G_PER_L CHEBI:30729
ethylenediaminetetraacetatoferrate(1-)

Preparation

  1. Bring to 1 liter with distilled water.
  2. Dilute 100 ml solution 1 to 1 litre with distilled water and then add 1 ml of solution 3, 66 ml solution 5 and 0.4 ml of solution 6. Dispense the medium into the growth vessels. If using sealed vessels it is appropriate to add 50% methane to the gas phase and autoclave at 121C (15psi) for 15 minutes. Autoclave the phosphate buffer separately. Filter sterilized the vitamin solution. When the growth medium is cool 1.0 ml/l vitamin solution and 10 ml/l of the phosphate buffer are added - if too warm the phosphate will precipitate. Liquid cultures should be grown with shaking.
  3. Dissolve the ingredients listed above (in that order) in about 700 ml of distilled water, and then make up to 1 litre.
  4. Dissolve in about 800 ml of water, adjust the pH to 6.8 and make up to 1 litre.
  5. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH).

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.861662Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1567
2026-01-28T19:48:19.548022Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T03:09:01.640241Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:00.701850Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001043
2026-03-17T06:30:07.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:12.336036+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHYLOPROFUNDUS SEDIMENTI MEDIUM" to "methyloprofundus_sedimenti_medium" for consistent matching
2026-06-05T07:41:33.365215+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 28 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.566223+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:07.646623+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.