anaerotignum_medium

CultureMech:001046 bacterial COMPLEX LIQUID
Original name: ANAEROTIGNUM MEDIUM

Overview

External ontology
mediadive.medium:156 (source link unavailable) — ANAEROTIGNUM MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 7.0, 'max': 7.2}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium156.pdf

Composition

flowchart LR
    CultureMech_001046["anaerotignum_medium"]
    ing0_L_Alanine["L-Alanine"]
    CultureMech_001046 --> ing0_L_Alanine
    CHEBI_16977["CHEBI:16977"]
    ing0_L_Alanine -.-> CHEBI_16977
    ing1_Peptone["Peptone"]
    CultureMech_001046 --> ing1_Peptone
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_001046 --> ing2_Yeast_extract
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001046 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001046 --> ing4_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing4_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing5_Potassium_phosphate_buffer["Potassium phosphate buffer"]
    CultureMech_001046 --> ing5_Potassium_phosphate_buffer
    ing6_CaSO4["CaSO4"]
    CultureMech_001046 --> ing6_CaSO4
    CHEBI_31346["CHEBI:31346"]
    ing6_CaSO4 -.-> CHEBI_31346
    ing7_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001046 --> ing7_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Sodium_resazurin -.-> CHEBI_8806
    ing8_NaHCO3["NaHCO3"]
    CultureMech_001046 --> ing8_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing8_NaHCO3 -.-> CHEBI_32139
    ing9_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_001046 --> ing9_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing9_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 L-Alanine
C3H7NO2 89.094 g/mol
3 G_PER_L CHEBI:16977
L-alanine
2 Peptone 3 G_PER_L
3 Yeast extract 4 G_PER_L
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.018 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
6 Potassium phosphate buffer 5 G_PER_L
7 CaSO4
Ca.O4S 136.141 g/mol
2.5 G_PER_L CHEBI:31346
calcium sulfate
8 Sodium resazurin
C12H7NO4 229.191 g/mol
0.0005 G_PER_L CHEBI:8806
Resazurin
9 NaHCO3
CHO3.Na 84.006 g/mol
1 G_PER_L CHEBI:32139
sodium hydrogencarbonate
10 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.3 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate

Preparation

  1. Dissolve ingredients (except bicarbonate and cysteine), adjust pH to 7.0, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add and dissolve bicarbonate and cysteine, then dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or seruim vials and autoclave. Adjust pH of complete medium to 7.0 - 7.2, if necessary.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:10.363426Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 156
2026-03-13T06:19:00.728674Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001046
2026-03-17T06:30:52.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:12.365990+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ANAEROTIGNUM MEDIUM" to "anaerotignum_medium" for consistent matching
2026-05-20T06:01:08.722919+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7-7.2' -> {'min': 7.0, 'max': 7.2}
2026-06-05T07:40:49.156896+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.335071+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.