Overview
- External ontology
- mediadive.medium:1581 (source link unavailable) — ALKALINE VOGESELLA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 8.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1581.pdf
Composition
flowchart LR
CultureMech_001056["alkaline_vogesella_medium"]
ing0_Glucose["Glucose"]
CultureMech_001056 --> ing0_Glucose
CHEBI_17234["CHEBI:17234"]
ing0_Glucose -.-> CHEBI_17234
ing1_Na_pyruvate["Na-pyruvate"]
CultureMech_001056 --> ing1_Na_pyruvate
CHEBI_50144["CHEBI:50144"]
ing1_Na_pyruvate -.-> CHEBI_50144
ing2_Casamino_acids["Casamino acids"]
CultureMech_001056 --> ing2_Casamino_acids
ing3_Peptone["Peptone"]
CultureMech_001056 --> ing3_Peptone
ing4_Yeast_extract["Yeast extract"]
CultureMech_001056 --> ing4_Yeast_extract
ing5_HEPES["HEPES"]
CultureMech_001056 --> ing5_HEPES
CHEBI_46756["CHEBI:46756"]
ing5_HEPES -.-> CHEBI_46756
ing6_KCl["KCl"]
CultureMech_001056 --> ing6_KCl
CHEBI_32588["CHEBI:32588"]
ing6_KCl -.-> CHEBI_32588
ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001056 --> ing7_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
ing8_NaCl["NaCl"]
CultureMech_001056 --> ing8_NaCl
CHEBI_26710["CHEBI:26710"]
ing8_NaCl -.-> CHEBI_26710
ing9_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001056 --> ing9_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing9_MgCl2_x_6_H2O -.-> CHEBI_86345
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glucose
C6H12O6 180.156 g/mol
|
0.5 G_PER_L |
CHEBI:17234
glucose
|
|
| 2 |
Na-pyruvate
C3H3O3.Na 110.044 g/mol
|
3 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 3 | Casamino acids | 0.5 G_PER_L | ||
| 4 | Peptone | 3 G_PER_L | ||
| 5 | Yeast extract | 3 G_PER_L | ||
| 6 | HEPES | 2.83 G_PER_L |
CHEBI:46756
HEPES
|
|
| 7 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 8 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.15 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 9 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 10 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.62 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Preparation
- Adjust pH to 8.0.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.901167Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1581 |
| 2026-01-28T19:48:29.870509Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:00.817934Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001056 |
| 2026-03-17T06:30:28.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:12.464418+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ALKALINE VOGESELLA MEDIUM" to "alkaline_vogesella_medium" for consistent matching |
| 2026-06-05T07:40:47.928696+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:35.578714+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:46:52.811298+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T13:57:59.694168+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |