Overview
- External ontology
- mediadive.medium:1582 (source link unavailable) — MELISSOCOCCUS PLUTONIUS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.6
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1582.pdf
Composition
flowchart LR
CultureMech_001057["melissococcus_plutonius_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_001057 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_Yeast_extract["Yeast extract"]
CultureMech_001057 --> ing1_Yeast_extract
ing2_Glucose["Glucose"]
CultureMech_001057 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_Starch["Starch"]
CultureMech_001057 --> ing3_Starch
CHEBI_28017["CHEBI:28017"]
ing3_Starch -.-> CHEBI_28017
ing4_Cysteine_hydrochloride["Cysteine hydrochloride"]
CultureMech_001057 --> ing4_Cysteine_hydrochloride
CHEBI_91248["CHEBI:91248"]
ing4_Cysteine_hydrochloride -.-> CHEBI_91248
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
13.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 | Yeast extract | 10 G_PER_L | ||
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:17234
glucose
|
|
| 4 | Starch | 10 G_PER_L |
CHEBI:28017
starch
|
|
| 5 |
Cysteine hydrochloride
C3H7NO2S.H2O.HCl 175.637 g/mol
|
1 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Preparation
- Adjust pH to 6.6 with NaOH. Boil medium and cool under N2, distribute under N2 and sterilize by autoclaving at 121°C for 20 minutes.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.903241Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1582 |
| 2026-03-13T06:19:00.822201Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001057 |
| 2026-03-17T06:30:25.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:12.469090+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MELISSOCOCCUS PLUTONIUS MEDIUM" to "melissococcus_plutonius_medium" for consistent matching |
| 2026-06-05T07:41:30.463203+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:06.571415+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |