bradymonas_sediminis_medium

CultureMech:001064 bacterial COMPLEX SOLID_AGAR
Original name: BRADYMONAS SEDIMINIS MEDIUM

Overview

External ontology
mediadive.medium:1588 (source link unavailable) — BRADYMONAS SEDIMINIS MEDIUM
Applications
  • Microbial cultivation
pH
7.8
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1588.pdf

Composition

flowchart LR
    CultureMech_001064["bradymonas_sediminis_medium"]
    ing0_Peptone["Peptone"]
    CultureMech_001064 --> ing0_Peptone
    ing1_Meat_extract["Meat extract"]
    CultureMech_001064 --> ing1_Meat_extract
    ing2_NaCl["NaCl"]
    CultureMech_001064 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_D____Trehalose["D(+)-Trehalose"]
    CultureMech_001064 --> ing3_D____Trehalose
    CHEBI_27082["CHEBI:27082"]
    ing3_D____Trehalose -.-> CHEBI_27082
    ing4_Agar["Agar"]
    CultureMech_001064 --> ing4_Agar
    CHEBI_2509["CHEBI:2509"]
    ing4_Agar -.-> CHEBI_2509
    ing5_Tap_water["Tap water"]
    CultureMech_001064 --> ing5_Tap_water
    CHEBI_15377["CHEBI:15377"]
    ing5_Tap_water -.-> CHEBI_15377
    ing6_Natural_sea_water["Natural sea water"]
    CultureMech_001064 --> ing6_Natural_sea_water
    ing7_KCl["KCl"]
    CultureMech_001064 --> ing7_KCl
    CHEBI_32588["CHEBI:32588"]
    ing7_KCl -.-> CHEBI_32588
    ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001064 --> ing8_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing9_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001064 --> ing9_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing9_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing10_NaHCO3["NaHCO3"]
    CultureMech_001064 --> ing10_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing10_NaHCO3 -.-> CHEBI_32139
    ing11_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001064 --> ing11_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing11_MgSO4_x_7_H2O -.-> CHEBI_31795

Ingredients (12)

# Ingredient Concentration Ontology Notes
1 Peptone 10 G_PER_L
2 Meat extract 3 G_PER_L
3 NaCl
Cl.Na 58.443 g/mol
33.129999999999995 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 5.0, 28.13]
4 D(+)-Trehalose
C12H22O11 342.297 g/mol
1 G_PER_L CHEBI:27082
trehalose
5 Agar 19 G_PER_L CHEBI:2509
agar
 (for solid medium)
6 Tap water
H2O 18.015 g/mol
250 G_PER_L CHEBI:15377
water
7 Natural sea water 750 G_PER_L
8 KCl
Cl.K 74.551 g/mol
0.77 G_PER_L CHEBI:32588
potassium chloride
9 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1.6 G_PER_L CHEBI:86158
calcium chloride dihydrate
10 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
4.8 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
11 NaHCO3
CHO3.Na 84.006 g/mol
0.11 G_PER_L CHEBI:32139
sodium hydrogencarbonate
12 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate

Preparation

  1. Dissolve beef extract and peptone by heating in tap water, adjust pH to 7.8 and boil for 10 min. Readjust pH to 7.3. Add agar and autoclave at 121°C for 20 min. Directly after autoclaving, add warm (55°C) sterile sea water. Liquid medium without agar should be combined when cooled to room temperature.
  2. Natural sea water is stored in the dark for at least three weeks to "age". If natural sea water is not available use artificial sea water:

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.923544Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1588
2026-03-13T03:08:59.784870Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:00.879615Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001064
2026-03-17T06:30:04.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:12.532911+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "BRADYMONAS SEDIMINIS MEDIUM" to "bradymonas_sediminis_medium" for consistent matching
2026-06-05T07:40:51.365698+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.