Overview
- External ontology
- mediadive.medium:1589 (source link unavailable) — modified BAP+ (GFS) MEDIUM FOR FRANKIA
- Applications
-
- Microbial cultivation
- pH
- 6.3
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1589.pdf
Composition
flowchart LR
CultureMech_001065["modified_bap_gfs_medium_for_frankia"]
ing0_KH2PO4["KH2PO4"]
CultureMech_001065 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_K2HPO4["K2HPO4"]
CultureMech_001065 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_NH4Cl["NH4Cl"]
CultureMech_001065 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_Na_propionate["Na-propionate"]
CultureMech_001065 --> ing3_Na_propionate
CHEBI_132106["CHEBI:132106"]
ing3_Na_propionate -.-> CHEBI_132106
ing4_Glucose["Glucose"]
CultureMech_001065 --> ing4_Glucose
CHEBI_17234["CHEBI:17234"]
ing4_Glucose -.-> CHEBI_17234
ing5_Fructose["Fructose"]
CultureMech_001065 --> ing5_Fructose
CHEBI_28757["CHEBI:28757"]
ing5_Fructose -.-> CHEBI_28757
ing6_Sodium_succinate_dibasic["Sodium succinate dibasic"]
CultureMech_001065 --> ing6_Sodium_succinate_dibasic
CHEBI_63675["CHEBI:63675"]
ing6_Sodium_succinate_dibasic -.-> CHEBI_63675
ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001065 --> ing7_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001065 --> ing8_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
ing9_Citric_acid["Citric acid"]
CultureMech_001065 --> ing9_Citric_acid
CHEBI_30769["CHEBI:30769"]
ing9_Citric_acid -.-> CHEBI_30769
ing10_Eisencitrat["Eisencitrat"]
CultureMech_001065 --> ing10_Eisencitrat
CHEBI_144434["CHEBI:144434"]
ing10_Eisencitrat -.-> CHEBI_144434
ing11_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_001065 --> ing11_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing11_CoSO4_x_7_H2O -.-> CHEBI_91244
ing12_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001065 --> ing12_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing12_CuSO4_x_5_H2O -.-> CHEBI_31440
ing13_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001065 --> ing13_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing13_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.95 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.6 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.27 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
Na-propionate
C3H5O2.Na 96.061 g/mol
|
0.48 G_PER_L |
CHEBI:132106
sodium propionate
|
|
| 5 |
Glucose
C6H12O6 180.156 g/mol
|
0.48 G_PER_L |
CHEBI:17234
glucose
|
|
| 6 | Fructose | 0.48 G_PER_L |
CHEBI:28757
fructose
|
|
| 7 |
Sodium succinate dibasic
C4H4O4.2Na 162.052 g/mol
|
0.48 G_PER_L |
CHEBI:63675
sodium succinate (anhydrous)
|
|
| 8 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.025 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 9 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.01 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 10 |
Citric acid
C6H8O7 192.123 g/mol
|
10 G_PER_L |
CHEBI:30769
citric acid
|
|
| 11 |
Eisencitrat
C6H5O7.Fe.H2O 262.959 g/mol
|
10 G_PER_L |
CHEBI:144434
iron(III) citrate monohydrate
|
|
| 12 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.01 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 13 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.8 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 14 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.25 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- The pH of the final solution is adjusted to 6.3.
- Note: Please autoclave the chelated iron solution before you add to the medium, so that solves everything!!!!
Solutions
Vitamine solution
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Thiamine HCl | 0.1 G_PER_L | |
| Nicotinic acid | 0.5 G_PER_L | |
| Pyridoxine hydrochloride | 0.5 G_PER_L |
Trace element solution
Dose: 0.1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| H3BO3 | 28.6 G_PER_L | |
| MnCl2 x 4 H2O | 18.1 G_PER_L | |
| ZnSO4 x 7 H2O | 2.2 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.926079Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1589 |
| 2026-03-13T06:19:00.888323Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001065 |
| 2026-03-17T06:30:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:12.542284+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "modified BAP+ (GFS) MEDIUM FOR FRANKIA" to "modified_bap_gfs_medium_for_frankia" for consistent matching |
| 2026-06-05T07:41:36.266977+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.542289+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-07T07:05:22.649824+00:00 | apply_cocktail_nesting.py | NESTED_FLATTENED_COCKTAIL | Moved 6 stock-strength component(s) out of ingredients into 2 solution(s): 3 -> 'Vitamine solution' @ 1 ml/l; 3 -> 'Trace element solution' @ 0.1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150). |