l_15b_tick_cell_medium (CultureMech:001068)

Overview

External ontology

mediadive.medium:1591 (source link unavailable) — L-15B Tick Cell Medium

Applications

Microbial cultivation

pH range

{'min': 5.5, 'max': 6.5}

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1591.pdf DSMZ Medium 1591 delegation verified against https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1591.pdf on 2026-08-25 (SHA-256 b4957b2953812ba39cb48d28097fe979b097355a3cc664c47462aac73902cf75); basal L-15B composition verified against https://www.dsmz.de/fileadmin/Bereiche/Microbiology/Dateien/Kultivierungshinweise/TickCellCultureMethods-4.pdf (SHA-256 be6881f2290a4d364b6de79d1aaa9f88378ca82e54394b055d0ea5b1e5df477a). Recipe encodes undiluted basal L-15B. L-15B300 dilution and optional FBS, TPB, lipoprotein, HEPES, and NaHCO3 supplements remain documented source variants, not base ingredients.

Composition

flowchart LR
    CultureMech_001068["l_15b_tick_cell_medium"]
    ing0_Cell_culture_grade_water["Cell culture grade water"]
    CultureMech_001068 --> ing0_Cell_culture_grade_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Cell_culture_grade_water -.-> CHEBI_15377
    ing1_Leibovitz_s_L_15_medium_powder["Leibovitz's L-15 medium powder"]
    CultureMech_001068 --> ing1_Leibovitz_s_L_15_medium_powder
    ing2_L_aspartic_acid["L-aspartic acid"]
    CultureMech_001068 --> ing2_L_aspartic_acid
    CHEBI_17053["CHEBI:17053"]
    ing2_L_aspartic_acid -.-> CHEBI_17053
    ing3_L_glutamine["L-glutamine"]
    CultureMech_001068 --> ing3_L_glutamine
    CHEBI_18050["CHEBI:18050"]
    ing3_L_glutamine -.-> CHEBI_18050
    ing4_L_proline["L-proline"]
    CultureMech_001068 --> ing4_L_proline
    CHEBI_17203["CHEBI:17203"]
    ing4_L_proline -.-> CHEBI_17203
    ing5_L_glutamic_acid["L-glutamic acid"]
    CultureMech_001068 --> ing5_L_glutamic_acid
    CHEBI_16015["CHEBI:16015"]
    ing5_L_glutamic_acid -.-> CHEBI_16015
    ing6_alpha_ketoglutaric_acid["alpha-ketoglutaric acid"]
    CultureMech_001068 --> ing6_alpha_ketoglutaric_acid
    CHEBI_30915["CHEBI:30915"]
    ing6_alpha_ketoglutaric_acid -.-> CHEBI_30915
    ing7_D_glucose["D-glucose"]
    CultureMech_001068 --> ing7_D_glucose
    CHEBI_17634["CHEBI:17634"]
    ing7_D_glucose -.-> CHEBI_17634

Ingredients (8)

#	Ingredient	Concentration	Ontology	Notes
1	Cell culture grade water H2O 18.015 g/mol	1 L	CHEBI:15377 water	Bring the basal medium to a final volume of 1 litre.
2	Leibovitz's L-15 medium powder	1 package VARIABLE		One package of GIBCO Leibovitz L-15 powder, Cat. No. 41300039. No defensible identity object is present in the reviewed MIM SSSOM.
3	L-aspartic acid C4H7NO4 133.103 g/mol	0.299 G_PER_L	CHEBI:17053 L-aspartic acid
4	L-glutamine C5H10N2O3 146.146 g/mol	0.292 G_PER_L	CHEBI:18050 L-glutamine
5	L-proline C5H9NO2 115.132 g/mol	0.300 G_PER_L	CHEBI:17203 L-proline
6	L-glutamic acid C5H9NO4 147.13 g/mol	0.490 G_PER_L	CHEBI:16015 L-glutamic acid
7	alpha-ketoglutaric acid C5H6O5 146.098 g/mol	0.299 G_PER_L	CHEBI:30915 2-oxoglutaric acid
8	D-glucose C6H12O6 180.156 g/mol	14.4105 G_PER_L	CHEBI:17634 D-glucose

Preparation

Use a volumetric flask dry-heat treated at 180 C or above for at least 2 hours to remove endotoxins.
Preload 500-700 ml sterile cell-culture-grade water, add components in the printed order, and bring the final volume to 1 litre.
Stir slowly for 1.5 hours, no longer, while protecting the medium from light.
Filter through a 0.22 micrometre filter into storage bottles.
Check a 4-5 ml aliquot from each bottle for sterility at 37 C. Confirm pH 5.5-6.5 and, if possible, 415 +/- 10 mOsm/L. Store protected from light at 4 C; the source states it keeps for months.

Solutions

L-15B mineral stock solution D

Dose: 1 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
Ascorbic acid	10.0 G_PER_L
Glutathione (reduced)	10.0 G_PER_L
FeSO4 x 7 H2O	0.50 G_PER_L
CoCl2 x 6 H2O	0.002 G_PER_L	Effective concentration in stock D: stock A contains 0.20 g/L and is added at 1 ml per 100 ml (10 ml/L). MIM maps the hydrated source label to cobalt dichloride.
CuSO4 x 5 H2O	0.002 G_PER_L	Effective concentration in stock D: stock A contains 0.20 g/L and is added at 10 ml/L.
MnSO4 x H2O	0.016 G_PER_L	Effective concentration in stock D: stock A contains 1.60 g/L and is added at 10 ml/L.
ZnSO4 x 7 H2O	0.020 G_PER_L	Effective concentration in stock D: stock A contains 2.00 g/L and is added at 10 ml/L.
Na2MoO4 x 2 H2O	0.002 G_PER_L	Effective concentration in stock D: stock B contains 0.20 g/L and is added at 10 ml/L.
Na2SeO3	0.002 G_PER_L	Effective concentration in stock D: stock C contains 0.20 g/L and is added at 10 ml/L.

The source prints 1000 mg ascorbic acid, 1000 mg reduced glutathione, and 50 mg FeSO4.7H2O per 100 ml, plus 1 ml each of stocks A-C. Because the schema does not support a stock nested inside another stock, A-C are represented above at their exact effective concentrations within D. Add 1 ml stock D per litre basal L-15B.

L-15B vitamin stock

Dose: 1 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
p-Aminobenzoic acid	1.00 G_PER_L
Cyanocobalamin	0.50 G_PER_L
Biotin	0.10 G_PER_L

The source prints 100, 50, and 10 mg per 100 ml, respectively. Add 1 ml vitamin stock per litre basal L-15B.

10 N NaOH solution

Dose: 0.5 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
NaOH	10 MOLAR	For monovalent NaOH, the source's 10 N concentration is 10 M.

Add 0.5 ml per litre basal L-15B.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:14.932685Z	mediadive-import	Imported from MediaDive	Source: DSMZ, ID: 1591
2026-02-03T11:26:47.446498	quality-tagger-v1.0	Added data quality flag	Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:19:00.910391Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:001068
2026-03-17T19:45:12.567460+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "L-15B Tick Cell Medium" to "l_15b_tick_cell_medium" for consistent matching
2026-08-06T07:56:36.107851+00:00	curate_placeholder_composition.py	REMOVED_PLACEHOLDER_INGREDIENT	Removed the 'See source for composition' placeholder ingredient; the record carries no imported composition and is flagged incomplete (#175).
2026-08-25T00:00:00-07:00	repair_dsmz_l15b.py	RESTORED_DSMZ_1591_L15B	Restored basal undiluted L-15B with mineral stock D, vitamin stock, NaOH stock, filtration, and source pH range. Stock masses printed per 100 ml were converted to per-litre concentrations; stocks A-C were materialized within D because the schema permits only one stock-composition level. identity objects were verified against the MIM SSSOM.