Overview
- External ontology
- mediadive.medium:1597a (source link unavailable) — METHANONATRONARCHAEUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 9.5
- High-metal flag
- True
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1597a.pdf
Composition
flowchart LR
CultureMech_001076["methanonatronarchaeum_medium"]
ing0_Na2CO3["Na2CO3"]
CultureMech_001076 --> ing0_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing0_Na2CO3 -.-> CHEBI_29377
ing1_NaHCO3["NaHCO3"]
CultureMech_001076 --> ing1_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing1_NaHCO3 -.-> CHEBI_32139
ing2_NaCl["NaCl"]
CultureMech_001076 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_K2HPO4["K2HPO4"]
CultureMech_001076 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_KCl["KCl"]
CultureMech_001076 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_NH4Cl["NH4Cl"]
CultureMech_001076 --> ing5_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing5_NH4Cl -.-> CHEBI_31206
ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001076 --> ing6_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
ing7_Methanol["Methanol"]
CultureMech_001076 --> ing7_Methanol
CHEBI_17790["CHEBI:17790"]
ing7_Methanol -.-> CHEBI_17790
ing8_Na_formate["Na-formate"]
CultureMech_001076 --> ing8_Na_formate
CHEBI_62965["CHEBI:62965"]
ing8_Na_formate -.-> CHEBI_62965
ing9_Na_acetate["Na-acetate"]
CultureMech_001076 --> ing9_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing9_Na_acetate -.-> CHEBI_32954
ing10_Yeast_extract["Yeast extract"]
CultureMech_001076 --> ing10_Yeast_extract
ing11_2_Mercaptoethanesulfonic_acid["2-Mercaptoethanesulfonic acid"]
CultureMech_001076 --> ing11_2_Mercaptoethanesulfonic_acid
CHEBI_17905["CHEBI:17905"]
ing11_2_Mercaptoethanesulfonic_acid -.-> CHEBI_17905
ing12_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001076 --> ing12_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing12_Na2S_x_9_H2O -.-> CHEBI_76209
ing13_EDTA["EDTA"]
CultureMech_001076 --> ing13_EDTA
CHEBI_4735["CHEBI:4735"]
ing13_EDTA -.-> CHEBI_4735
ing14_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001076 --> ing14_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing14_FeSO4_x_7_H2O -.-> CHEBI_75836
ing15_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001076 --> ing15_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing15_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing16_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001076 --> ing16_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing16_MnCl2_x_4_H2O -.-> CHEBI_86368
ing17_H3BO3["H3BO3"]
CultureMech_001076 --> ing17_H3BO3
CHEBI_33118["CHEBI:33118"]
ing17_H3BO3 -.-> CHEBI_33118
ing18_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001076 --> ing18_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing18_CoCl2_x_6_H2O -.-> CHEBI_53503
ing19_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001076 --> ing19_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing19_CuCl2_x_2_H2O -.-> CHEBI_86318
ing20_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001076 --> ing20_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing20_NiCl2_x_6_H2O -.-> CHEBI_34887
ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001076 --> ing21_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing22_NaOH["NaOH"]
CultureMech_001076 --> ing22_NaOH
CHEBI_32145["CHEBI:32145"]
ing22_NaOH -.-> CHEBI_32145
ing23_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_001076 --> ing23_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing23_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing24_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001076 --> ing24_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing24_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing25_Biotin["Biotin"]
CultureMech_001076 --> ing25_Biotin
CHEBI_15956["CHEBI:15956"]
ing25_Biotin -.-> CHEBI_15956
ing26_Folic_acid["Folic acid"]
CultureMech_001076 --> ing26_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing26_Folic_acid -.-> CHEBI_27470
ing27_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001076 --> ing27_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing27_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing28_Thiamine_HCl["Thiamine HCl"]
CultureMech_001076 --> ing28_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing28_Thiamine_HCl -.-> CHEBI_49105
ing29_Riboflavin["Riboflavin"]
CultureMech_001076 --> ing29_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing29_Riboflavin -.-> CHEBI_17015
CultureMech_001076 --> more["...5 more"]
Ingredients (35)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2CO3
CO3.2Na 105.988 g/mol
|
192.708 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 2 |
NaHCO3
CHO3.Na 84.006 g/mol
|
36.4583 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
266.6667 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 16.6667, 250.0] |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
3.6458399999999997 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
[Merged 2 duplicates: 1.04167, 2.60417] |
| 5 |
KCl
Cl.K 74.551 g/mol
|
5.20833 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 6 |
NH4Cl
Cl.H4N 53.492 g/mol
|
40 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 7 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
50 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 8 |
Methanol
CH4O 32.042 g/mol
|
792 G_PER_L |
CHEBI:17790
methanol
|
|
| 9 |
Na-formate
CHO2.Na 68.007 g/mol
|
340 G_PER_L |
CHEBI:62965
sodium formate
|
|
| 10 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
16 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 11 | Yeast extract | 4 G_PER_L | ||
| 12 |
2-Mercaptoethanesulfonic acid
C2H6O3S2 142.201 g/mol
|
15 G_PER_L |
CHEBI:17905
coenzyme M
|
|
| 13 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
148.0 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
[Merged 2 duplicates: 25.0, 123.0] |
| 14 |
EDTA
C10H16N2O8 292.244 g/mol
|
5 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
|
| 15 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
156.2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
[Merged 2 duplicates: 2.2, 154.0] |
| 16 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 17 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 18 |
H3BO3
H3BO3 61.833 g/mol
|
0.03 G_PER_L |
CHEBI:33118
boric acid
|
|
| 19 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 20 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.03 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 21 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 22 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 23 |
NaOH
HO.Na 39.997 g/mol
|
0.5 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 24 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 25 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 26 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 27 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 28 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 29 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 30 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 31 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 32 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 33 | Vitamin B12 | 0.001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 34 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 35 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Sterilize solutions A and B in closed thick-walled screw-top bottles (e.g., SCHOTT) for 20 min at 120°C. The pH of solution A after sterilization should be 10. There is some precipitate forming that settles at the bottom after 3 - 4 days. It is best to remove precipitates by decantation before using solution A for medium preparation.
- Combine solution A with solution B and sparge medium with 100% N2 gas for at least 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solutions C to L are sterilized separately under 100% N2 gas. Vitamins and coenzyme M should be sterilized by filtration. To complete the medium appropriate amounts of solutions C to L are added to the combined sterile solutions A and B in the sequence as indicated. Final pH of the medium should be 9.5.
- Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth at the beginning. For transfers use 5 - 10% inoculum.
- pH 3.0-4.0
- Heat distilled water to 50°C in a 250 ml beaker with a stir bar present. While rapidly stirring the water, add the ferrous sulfate followed immediately by the sodium sulfide. The formed black FeS sludge is decanted into a glass bottle that can be stoppered. The FeS is allowed to settle for several hours and then the overlying water is decanted and replaced. This procedure is repeated at least five times to wash the FeS. After washing, the pH of the FeS solution should be close to neutrality. The FeS suspension can be kept in closed bottles or tubes under a nitrogen atmosphere for at least three months.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.955788Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1597a |
| 2026-02-06T05:10:27.952531Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T03:09:02.327219Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:19:00.986097Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001076 |
| 2026-03-17T06:30:07.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:12.650934+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHANONATRONARCHAEUM MEDIUM" to "methanonatronarchaeum_medium" for consistent matching |
| 2026-06-05T07:41:32.082061+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 33 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:07.179886+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |