Overview
- External ontology
- mediadive.medium:1606 (source link unavailable) — RUBROBACTER CARBOXYDOVORANS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 8.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1606.pdf
Composition
flowchart LR
CultureMech_001087["rubrobacter_carboxydovorans_medium"]
ing0_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
CultureMech_001087 --> ing0_Na2HPO4_x_2_H2O
CHEBI_91258["CHEBI:91258"]
ing0_Na2HPO4_x_2_H2O -.-> CHEBI_91258
ing1_KH2PO4["KH2PO4"]
CultureMech_001087 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001087 --> ing2_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing2_MnSO4_x_H2O -.-> CHEBI_86364
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001087 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001087 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_Ferric_ammonium_citrate["Ferric ammonium citrate"]
CultureMech_001087 --> ing5_Ferric_ammonium_citrate
CHEBI_31604["CHEBI:31604"]
ing5_Ferric_ammonium_citrate -.-> CHEBI_31604
ing6_Yeast_extract["Yeast extract"]
CultureMech_001087 --> ing6_Yeast_extract
ing7_Tryptone["Tryptone"]
CultureMech_001087 --> ing7_Tryptone
ing8_Agar["Agar"]
CultureMech_001087 --> ing8_Agar
CHEBI_2509["CHEBI:2509"]
ing8_Agar -.-> CHEBI_2509
ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001087 --> ing9_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001087 --> ing10_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
ing11_H3BO3["H3BO3"]
CultureMech_001087 --> ing11_H3BO3
CHEBI_33118["CHEBI:33118"]
ing11_H3BO3 -.-> CHEBI_33118
ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001087 --> ing12_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001087 --> ing13_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
ing14_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001087 --> ing14_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing14_NiCl2_x_6_H2O -.-> CHEBI_34887
ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001087 --> ing15_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
|
4.5 G_PER_L |
CHEBI:91258
disodium hydrogenphosphate dihydrate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.01 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.01 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 | Ferric ammonium citrate | 0.005 G_PER_L |
CHEBI:31604
ferric ammonium citrate
|
|
| 7 | Yeast extract | 1 G_PER_L | ||
| 8 | Tryptone | 1 G_PER_L | ||
| 9 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
| 10 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 11 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 12 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
|
| 13 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 14 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 15 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 16 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Adjust pH to 8.0. Agar if necessary 15.0 g/l. Incubate without agitation at 50°C.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.993528Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1606 |
| 2026-03-13T06:19:01.099272Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001087 |
| 2026-03-17T06:30:33.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:12.780520+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RUBROBACTER CARBOXYDOVORANS MEDIUM" to "rubrobacter_carboxydovorans_medium" for consistent matching |
| 2026-06-05T07:41:44.756807+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.113144+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |