Overview
- External ontology
- mediadive.medium:1608 (source link unavailable) — METHYLOPHILA MEDIUM (YIM 38)
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1608.pdf
Composition
flowchart LR
CultureMech_001089["methylophila_medium_yim_38"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_001089 --> ing0_Yeast_extract
ing1_Malt_extract["Malt extract"]
CultureMech_001089 --> ing1_Malt_extract
ing2_Agar["Agar"]
CultureMech_001089 --> ing2_Agar
CHEBI_2509["CHEBI:2509"]
ing2_Agar -.-> CHEBI_2509
ing3_Glucose["Glucose"]
CultureMech_001089 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_001089 --> ing4_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing4_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing5_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001089 --> ing5_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing5_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing6_Riboflavin["Riboflavin"]
CultureMech_001089 --> ing6_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing6_Riboflavin -.-> CHEBI_17015
ing7_Nicotinic_acid["Nicotinic acid"]
CultureMech_001089 --> ing7_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing7_Nicotinic_acid -.-> CHEBI_15940
ing8_L_Phenylalanine["L-Phenylalanine"]
CultureMech_001089 --> ing8_L_Phenylalanine
CHEBI_17295["CHEBI:17295"]
ing8_L_Phenylalanine -.-> CHEBI_17295
ing9_Biotin["Biotin"]
CultureMech_001089 --> ing9_Biotin
CHEBI_15956["CHEBI:15956"]
ing9_Biotin -.-> CHEBI_15956
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 4 G_PER_L | ||
| 2 | Malt extract | 5 G_PER_L | ||
| 3 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
|
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
4 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.0002 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 6 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.0002 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 7 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.0002 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 8 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.0002 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 9 |
L-Phenylalanine
C9H11NO2 165.192 g/mol
|
0.0002 G_PER_L |
CHEBI:17295
L-phenylalanine
|
|
| 10 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.0002 G_PER_L |
CHEBI:15956
biotin
|
Preparation
- Sterilize 20 min./121°C. After cooling to 60°C add to the medium:
- Adjust pH to 7.5
- Store in the dark and cold (5°C).
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.997545Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1608 |
| 2026-03-13T06:19:01.108469Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001089 |
| 2026-03-17T06:29:58.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:12.791579+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHYLOPHILA MEDIUM (YIM 38)" to "methylophila_medium_yim_38" for consistent matching |
| 2026-06-05T07:41:33.351552+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |