Overview
- External ontology
- mediadive.medium:1609 (source link unavailable) — HEART INFUSION BLOOD AGAR
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.0, 'max': 7.4}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1609.pdf
Composition
flowchart LR
CultureMech_001090["heart_infusion_blood_agar"]
ing0_Bacto_Heart_Infusion_Broth["Bacto Heart Infusion Broth"]
CultureMech_001090 --> ing0_Bacto_Heart_Infusion_Broth
ing1_Agar["Agar"]
CultureMech_001090 --> ing1_Agar
CHEBI_2509["CHEBI:2509"]
ing1_Agar -.-> CHEBI_2509
ing2_Defibrinated_Blood["Defibrinated Blood"]
CultureMech_001090 --> ing2_Defibrinated_Blood
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Bacto Heart Infusion Broth | 25 G_PER_L | ||
| 2 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
|
| 3 | Defibrinated Blood | 75 G_PER_L |
Preparation
- Allow to soak for 10 minutes, swirl to mix then sterilize by autoclaving at 121°C for 15 minutes. Cool to 47°C then aseptically add 5-10% of sterile defibrinated blood (horse or sheep), mix well and pour plates. pH: 7.2 ± 0.2
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.999606Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1609 |
| 2026-03-13T06:19:01.112124Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001090 |
| 2026-03-17T06:29:42.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:12.795742+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HEART INFUSION BLOOD AGAR" to "heart_infusion_blood_agar" for consistent matching |
| 2026-05-20T06:01:22.455439+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7-7.4' -> {'min': 7.0, 'max': 7.4} |
| 2026-06-05T07:41:08.341669+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-13T00:00:00-07:00 | repair_remaining_score10_grounding_batch9.py | RESOLVED_REMAINING_SCORE10_GROUNDING_BATCH9 | Reviewed remaining score-10 grounding rows: Bacto Heart Infusion Broth, Defibrinated Blood. |