Overview
- External ontology
- mediadive.medium:160 (source link unavailable) — CYTOPHAGA HUTCHINSONII MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium160.pdf
Composition
flowchart LR
CultureMech_001091["cytophaga_hutchinsonii_medium"]
ing0_Casitone["Casitone"]
CultureMech_001091 --> ing0_Casitone
ing1_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001091 --> ing1_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing1_CaCl2_x_2_H2O -.-> CHEBI_86158
ing2_Yeast_extract["Yeast extract"]
CultureMech_001091 --> ing2_Yeast_extract
ing3_Agar["Agar"]
CultureMech_001091 --> ing3_Agar
CHEBI_2509["CHEBI:2509"]
ing3_Agar -.-> CHEBI_2509
ing4_Cellobiose["Cellobiose"]
CultureMech_001091 --> ing4_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing4_Cellobiose -.-> CHEBI_17057
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Casitone | 3 G_PER_L | ||
| 2 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.36 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 3 | Yeast extract | 1 G_PER_L | ||
| 4 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(alternative) |
| 5 |
Cellobiose
C12H22O11 342.297 g/mol
|
5 G_PER_L |
CHEBI:17057
cellobiose
|
(alternative) |
Preparation
- Adjust pH to 7.2.
- To medium add 0.5% cellobiose (filter-sterilized) or distribute medium (without agar) in 5 ml amounts in test tubes, add a strip of filter paper (7 cm long) to each and autoclave, or place sterile pieces of filter paper on the surface of agarized medium.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.371048Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 160 |
| 2026-03-13T06:19:01.115530Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001091 |
| 2026-03-17T06:30:02.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:12.799434+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CYTOPHAGA HUTCHINSONII MEDIUM" to "cytophaga_hutchinsonii_medium" for consistent matching |
| 2026-06-05T07:40:55.223520+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-10-07T10:55:41.454416+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |