basic_mineral_medium

CultureMech:001096 bacterial DEFINED LIQUID
Original name: Basic mineral medium

Overview

External ontology
mediadive.medium:1615 (source link unavailable) — Basic mineral medium
Applications
  • Microbial cultivation
pH range
{'min': 7.0, 'max': 7.2}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1615.pdf

Composition

flowchart LR
    CultureMech_001096["basic_mineral_medium"]
    ing0_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001096 --> ing0_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing0_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing1_Sodium_acetate["Sodium acetate"]
    CultureMech_001096 --> ing1_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing1_Sodium_acetate -.-> CHEBI_32954
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001096 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_Na_aspartate["Na-aspartate"]
    CultureMech_001096 --> ing3_Na_aspartate
    ing4_L_Methionine["L-Methionine"]
    CultureMech_001096 --> ing4_L_Methionine
    CHEBI_16643["CHEBI:16643"]
    ing4_L_Methionine -.-> CHEBI_16643
    ing5_EDTA["EDTA"]
    CultureMech_001096 --> ing5_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing5_EDTA -.-> CHEBI_4735
    ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001096 --> ing6_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing6_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing7_ZnSO4["ZnSO4"]
    CultureMech_001096 --> ing7_ZnSO4
    CHEBI_35176["CHEBI:35176"]
    ing7_ZnSO4 -.-> CHEBI_35176
    ing8_MnCl2["MnCl2"]
    CultureMech_001096 --> ing8_MnCl2
    CHEBI_63041["CHEBI:63041"]
    ing8_MnCl2 -.-> CHEBI_63041
    ing9_H3BO3["H3BO3"]
    CultureMech_001096 --> ing9_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing9_H3BO3 -.-> CHEBI_33118
    ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001096 --> ing10_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing11_CuCl2_x_5_H2O["CuCl2 x 5 H2O"]
    CultureMech_001096 --> ing11_CuCl2_x_5_H2O
    CHEBI_91245["CHEBI:91245"]
    ing11_CuCl2_x_5_H2O -.-> CHEBI_91245
    ing12_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001096 --> ing12_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing12_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing13_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001096 --> ing13_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing13_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing14_K2HPO4["K2HPO4"]
    CultureMech_001096 --> ing14_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing14_K2HPO4 -.-> CHEBI_131527
    ing15_KH2PO4["KH2PO4"]
    CultureMech_001096 --> ing15_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing15_KH2PO4 -.-> CHEBI_63036
    ing16_Thiamine["Thiamine"]
    CultureMech_001096 --> ing16_Thiamine
    CHEBI_18385["CHEBI:18385"]
    ing16_Thiamine -.-> CHEBI_18385
    ing17_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_001096 --> ing17_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing17_Calcium_pantothenate -.-> CHEBI_31345
    ing18_Biotin["Biotin"]
    CultureMech_001096 --> ing18_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing18_Biotin -.-> CHEBI_15956
    ing19_PABA["PABA"]
    CultureMech_001096 --> ing19_PABA
    CHEBI_30753["CHEBI:30753"]
    ing19_PABA -.-> CHEBI_30753
    ing20_Nicotinic_acid["Nicotinic acid"]
    CultureMech_001096 --> ing20_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing20_Nicotinic_acid -.-> CHEBI_15940
    ing21_Pyridoxine["Pyridoxine"]
    CultureMech_001096 --> ing21_Pyridoxine
    CHEBI_16709["CHEBI:16709"]
    ing21_Pyridoxine -.-> CHEBI_16709
    ing22_NaOH["NaOH"]
    CultureMech_001096 --> ing22_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing22_NaOH -.-> CHEBI_32145
    ing23_Folic_acid["Folic acid"]
    CultureMech_001096 --> ing23_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing23_Folic_acid -.-> CHEBI_27470
    ing24_Riboflavin["Riboflavin"]
    CultureMech_001096 --> ing24_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing24_Riboflavin -.-> CHEBI_17015
    ing25_B12["B12"]
    CultureMech_001096 --> ing25_B12

Ingredients (26)

# Ingredient Concentration Ontology Notes
1 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.246476 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
2 Sodium acetate
C2H3O2.Na 82.034 g/mol
0.8203 G_PER_L CHEBI:32954
sodium acetate
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.05 G_PER_L CHEBI:86158
calcium chloride dihydrate
4 Na-aspartate 0.35414 G_PER_L
5 L-Methionine
C5H11NO2S 149.215 g/mol
0.0074605 G_PER_L CHEBI:16643
L-methionine
6 EDTA
C10H16N2O8 292.244 g/mol
5 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
7 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
8 ZnSO4
O4S.Zn 161.453 g/mol
0.1 G_PER_L CHEBI:35176
zinc sulfate
9 MnCl2
Cl2Mn 125.844 g/mol
0.03 G_PER_L CHEBI:63041
manganese(II) chloride
10 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
11 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
12 CuCl2 x 5 H2O
Cl2Cu.5H2O 224.527 g/mol
0.01 G_PER_L CHEBI:91245
copper(II) chloride pentahydrate
13 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
14 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.02 G_PER_L CHEBI:75213
sodium molybdate dihydrate
15 K2HPO4
HO4P.2K 174.174 g/mol
2.26429 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
16 KH2PO4
H2O4P.K 136.084 g/mol
0.952595 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
17 Thiamine
C12H17N4OS 265.362 g/mol
0.05 G_PER_L CHEBI:18385
thiamine(1+)
18 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.05 G_PER_L CHEBI:31345
Calcium pantothenate
19 Biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
20 PABA
C7H7NO2 137.138 g/mol
0.05 G_PER_L CHEBI:30753
4-aminobenzoic acid
21 Nicotinic acid
C6H5NO2 123.111 g/mol
0.05 G_PER_L CHEBI:15940
nicotinic acid
22 Pyridoxine
C8H11NO3 169.18 g/mol
0.1 G_PER_L CHEBI:16709
pyridoxine
23 NaOH
HO.Na 39.997 g/mol
100 G_PER_L CHEBI:32145
sodium hydroxide
24 Folic acid
C19H19N7O6 441.404 g/mol
0.02 G_PER_L CHEBI:27470
folic acid
25 Riboflavin
C17H20N4O6 376.369 g/mol
0.05 G_PER_L CHEBI:17015
riboflavin
26 B12 0.005 G_PER_L

Preparation

  1. pH 7.0-7.2
  2. After sterilization add from sterile stock solutions:
  3. might result in colloid formation, especially when added into hot medium, but dissolves after vigorous shaking
  4. Adjust pH to 3.0-4.0 with HCl.
  5. Autoclave at 121 °C for 15 min.
  6. Note: autoclave in closed bottles
  7. Sterilize at 121°C for 20 minutes in 1L Schott bottle
  8. Sterilize by filtration.
  9. Mix the four solutions in the ratio 1:1:1:1.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.012799Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1615
2026-02-06T05:10:36.256938Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:19:01.156316Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001096
2026-03-17T06:30:22.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:12.843418+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Basic mineral medium" to "basic_mineral_medium" for consistent matching
2026-05-20T06:01:09.656681+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7-7.2' -> {'min': 7.0, 'max': 7.2}
2026-06-05T07:40:50.600884+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.819823+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.