rugosibacter_medium

CultureMech:001103 bacterial DEFINED LIQUID
Original name: RUGOSIBACTER MEDIUM

Overview

External ontology
mediadive.medium:1622 (source link unavailable) — RUGOSIBACTER MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 6.8, 'max': 7.2}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1622.pdf

Composition

flowchart LR
    CultureMech_001103["rugosibacter_medium"]
    ing0_Vitamin_B12["Vitamin B12"]
    CultureMech_001103 --> ing0_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing0_Vitamin_B12 -.-> CHEBI_176843
    ing1_KH2PO4["KH2PO4"]
    CultureMech_001103 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
    CultureMech_001103 --> ing2_Na2HPO4_x_2_H2O
    CHEBI_91258["CHEBI:91258"]
    ing2_Na2HPO4_x_2_H2O -.-> CHEBI_91258
    ing3_NH4NO3["NH4NO3"]
    CultureMech_001103 --> ing3_NH4NO3
    CHEBI_63038["CHEBI:63038"]
    ing3_NH4NO3 -.-> CHEBI_63038
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001103 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001103 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_Na_pyruvate["Na-pyruvate"]
    CultureMech_001103 --> ing6_Na_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing6_Na_pyruvate -.-> CHEBI_50144
    ing7_HCl["HCl"]
    CultureMech_001103 --> ing7_HCl
    CHEBI_17883["CHEBI:17883"]
    ing7_HCl -.-> CHEBI_17883
    ing8_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_001103 --> ing8_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing8_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing9_ZnCl2["ZnCl2"]
    CultureMech_001103 --> ing9_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing9_ZnCl2 -.-> CHEBI_49976
    ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001103 --> ing10_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing11_H3BO3["H3BO3"]
    CultureMech_001103 --> ing11_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing11_H3BO3 -.-> CHEBI_33118
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001103 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001103 --> ing13_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing14_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001103 --> ing14_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing14_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001103 --> ing15_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (16)

# Ingredient Concentration Ontology Notes
1 Vitamin B12 1 G_PER_L CHEBI:176843
vitamin B12
2 KH2PO4
H2O4P.K 136.084 g/mol
0.24 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
1.44 G_PER_L CHEBI:91258
disodium hydrogenphosphate dihydrate
4 NH4NO3
H4N.NO3 80.043 g/mol
0.8 G_PER_L CHEBI:63038
ammonium nitrate
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.48 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.3 G_PER_L CHEBI:86158
calcium chloride dihydrate
7 Na-pyruvate
C3H3O3.Na 110.044 g/mol
4 G_PER_L CHEBI:50144
sodium pyruvate
8 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
9 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
10 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
11 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
12 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
13 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
14 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
15 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
16 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Adjust solutions A and B to pH 7.0±0.2, autoclave separately and combine after cooling.
  2. Add 1.0 ml sterile vitamin B12 solution (50 mg/l) per litre.
  3. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.029409Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1622
2026-01-28T19:48:43.302094Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:01.212005Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001103
2026-03-17T06:30:57.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:12.905664+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "RUGOSIBACTER MEDIUM" to "rugosibacter_medium" for consistent matching
2026-05-20T06:02:04.426937+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '6.8-7.2' -> {'min': 6.8, 'max': 7.2}
2026-06-05T07:41:44.766709+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.116768+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.