Overview
- External ontology
- mediadive.medium:1628 (source link unavailable) — M408 MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1628.pdf
Composition
flowchart LR
CultureMech_001107["m408_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_001107 --> ing0_Yeast_extract
ing1_Mannitol["Mannitol"]
CultureMech_001107 --> ing1_Mannitol
CHEBI_29864["CHEBI:29864"]
ing1_Mannitol -.-> CHEBI_29864
ing2_K2HPO4["K2HPO4"]
CultureMech_001107 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001107 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_NaCl["NaCl"]
CultureMech_001107 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_Agar["Agar"]
CultureMech_001107 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 1 G_PER_L | ||
| 2 |
Mannitol
C6H14O6 182.172 g/mol
|
10 G_PER_L |
CHEBI:29864
mannitol
|
|
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
0.1 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 6 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Adjust pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.040018Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1628 |
| 2026-03-13T06:19:01.228511Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001107 |
| 2026-03-17T06:29:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:12.924727+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "M408 MEDIUM" to "m408_medium" for consistent matching |
| 2026-06-05T07:41:11.861051+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.708582+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T13:57:59.694168+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |