synthetic_crenarchaeota_medium_scm

Overview

External ontology

mediadive.medium:1630e (source link unavailable) — Synthetic Crenarchaeota Medium (SCM)

Applications

• Microbial cultivation

pH

7.6

Notes

Source: DSMZ

Composition

flowchart LR
    CultureMech_001114["synthetic_crenarchaeota_medium_scm"]
    ing0_NaCl["NaCl"]
    CultureMech_001114 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001114 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001114 --> ing2_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001114 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_KBr["KBr"]
    CultureMech_001114 --> ing4_KBr
    CHEBI_32030["CHEBI:32030"]
    ing4_KBr -.-> CHEBI_32030
    ing5_Double_distilled_water["Double distilled water"]
    CultureMech_001114 --> ing5_Double_distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing5_Double_distilled_water -.-> CHEBI_15377
    ing6_NaOH["NaOH"]
    CultureMech_001114 --> ing6_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing6_NaOH -.-> CHEBI_32145
    ing7_HEPES["HEPES"]
    CultureMech_001114 --> ing7_HEPES
    CHEBI_46756["CHEBI:46756"]
    ing7_HEPES -.-> CHEBI_46756
    ing8_HCl["HCl"]
    CultureMech_001114 --> ing8_HCl
    CHEBI_17883["CHEBI:17883"]
    ing8_HCl -.-> CHEBI_17883
    ing9_H3BO3["H3BO3"]
    CultureMech_001114 --> ing9_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing9_H3BO3 -.-> CHEBI_33118
    ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001114 --> ing10_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001114 --> ing11_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing12_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001114 --> ing12_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing12_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001114 --> ing13_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing14_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001114 --> ing14_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing14_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001114 --> ing15_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing16_FeNa_EDTA["FeNa-EDTA"]
    CultureMech_001114 --> ing16_FeNa_EDTA
    ing17_NaHCO3["NaHCO3"]
    CultureMech_001114 --> ing17_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing17_NaHCO3 -.-> CHEBI_32139
    ing18_NH4Cl["NH4Cl"]
    CultureMech_001114 --> ing18_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing18_NH4Cl -.-> CHEBI_31206
    ing19_KH2PO4["KH2PO4"]
    CultureMech_001114 --> ing19_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing19_KH2PO4 -.-> CHEBI_63036
    ing20_alpha_ketoglutamate["alpha-ketoglutamate"]
    CultureMech_001114 --> ing20_alpha_ketoglutamate

Ingredients (21)

Preparation

1. Incubate all used glass ware and magnetic stirring rods in 0.1 M HCl and rinse 3x with double distilled water to remove residual detergents (always check visual for soap bubbles).
2. Autoclave the basic salt water medium and cool to room temperature. This medium should be free of any precipitations!
3. Aseptically, add per L of basic salt water medium the following solutions from sterile stocks (do not store complemented SCM): Trace element solution (Nitrososphaera), FeNaEDTA solution, NaHCO3 solution, HEPES buffer solution, NH4Cl solution, KH2PO4 solution
4. Adjust pH to 7.6.
5. Fist dissolve NaOH in ca 600 ml water, then add HEPES, adjust pH to 7.5 and fill up to 1000 ml volume.
6. This solution contains no iron! Sterilise by autoclaving or filtration (0.2 µm) and store in the dark at 4 °C.
7. Sterilise by filtration (0.2 µm) and store in the dark at 4 °C.
8. Dissolve 4.2 g of NaHCO3 in 50 mL Double distilled water (use a small beaker with stirring rod to dissolve) and sterilize by filtration (0.2 µm) in the laminar flow hood. Store in a sterile 50 mL falcon tube at 4 °C. Avoid high headspace to liquid ratios to ensure stable concentration.
9. Dissolve 2.67 g ammonium chloride in 50 mL Double distilled water.
10. Filtrate (0.2 µm).

Target organisms

No target organisms are recorded.

Provenance