Overview
- External ontology
- mediadive.medium:1640 (source link unavailable) — METHANOMASSILIICOCCUS ALVUS MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.4, 'max': 7.6}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1640.pdf
Composition
flowchart LR
CultureMech_001125["methanomassiliicoccus_alvus_medium"]
ing0_Anaerobe_Basal_Broth_CM0957["Anaerobe Basal Broth CM0957"]
CultureMech_001125 --> ing0_Anaerobe_Basal_Broth_CM0957
ing1_Methanol["Methanol"]
CultureMech_001125 --> ing1_Methanol
CHEBI_17790["CHEBI:17790"]
ing1_Methanol -.-> CHEBI_17790
ing2_Sodium_resazurin["Sodium resazurin"]
CultureMech_001125 --> ing2_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing2_Sodium_resazurin -.-> CHEBI_8806
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Anaerobe Basal Broth CM0957 | 35.0495 G_PER_L | ||
| 2 |
Methanol
CH4O 32.042 g/mol
|
7.84158 G_PER_L |
CHEBI:17790
methanol
|
|
| 3 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.00049505 G_PER_L |
CHEBI:8806
Resazurin
|
Preparation
- Suspend Anaerobe Basal Broth in distilled water. Adjust pH to 7.4 with 1N NaOH and bring to the boil to dissolve ingredients completely. Then cool medium to room temperature while sparging with 100% N2 gas to make it anoxic. Distribute medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. After sterilization add methanol from an anoxic stock solution autoclaved under 100% N2 gas atmosphere. Adjust pH of complete medium to 7.4 - 7.6, if necessary.
- After inoculation, add sterile 80% H2 and 20% CO2 gas mixture to 0.5 bar overpressure.
- Note: Use 10% (v/v) inoculum.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.100089Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1640 |
| 2026-03-13T06:19:01.414466Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001125 |
| 2026-03-17T06:30:45.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:13.128296+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHANOMASSILIICOCCUS ALVUS MEDIUM" to "methanomassiliicoccus_alvus_medium" for consistent matching |
| 2026-05-20T06:01:55.952986+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7.4-7.6' -> {'min': 7.4, 'max': 7.6} |
| 2026-06-05T07:41:31.918874+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |