Overview
- External ontology
- mediadive.medium:1641 (source link unavailable) — DOUBLE CONCENTRATED 830 + 0.06% SODIUM PYRUVATE
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1641.pdf
Composition
flowchart LR
CultureMech_001126["double_concentrated_830_0_06_sodium_pyruvate"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_001126 --> ing0_Yeast_extract
ing1_Proteose_peptone["Proteose peptone"]
CultureMech_001126 --> ing1_Proteose_peptone
ing2_Casamino_acids["Casamino acids"]
CultureMech_001126 --> ing2_Casamino_acids
ing3_Glucose["Glucose"]
CultureMech_001126 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Starch["Starch"]
CultureMech_001126 --> ing4_Starch
CHEBI_28017["CHEBI:28017"]
ing4_Starch -.-> CHEBI_28017
ing5_Na_pyruvate["Na-pyruvate"]
CultureMech_001126 --> ing5_Na_pyruvate
CHEBI_50144["CHEBI:50144"]
ing5_Na_pyruvate -.-> CHEBI_50144
ing6_K2HPO4["K2HPO4"]
CultureMech_001126 --> ing6_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing6_K2HPO4 -.-> CHEBI_131527
ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001126 --> ing7_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
ing8_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_001126 --> ing8_Sodium_pyruvate
ing8_Sodium_pyruvate -.-> CHEBI_50144
ing9_Agar["Agar"]
CultureMech_001126 --> ing9_Agar
CHEBI_2509["CHEBI:2509"]
ing9_Agar -.-> CHEBI_2509
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 1 G_PER_L | ||
| 2 | Proteose peptone | 1 G_PER_L | ||
| 3 | Casamino acids | 1 G_PER_L | ||
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 | Starch | 1 G_PER_L |
CHEBI:28017
starch
|
|
| 6 |
Na-pyruvate
C3H3O3.Na 110.044 g/mol
|
0.6 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 7 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.6 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 8 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 9 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
0.6 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 10 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.101692Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1641 |
| 2026-01-28T19:48:24.845741Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:01.419424Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001126 |
| 2026-03-17T06:30:17.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:13.133503+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DOUBLE CONCENTRATED 830 + 0.06% SODIUM PYRUVATE" to "double_concentrated_830_0_06_sodium_pyruvate" for consistent matching |
| 2026-06-05T07:40:58.807708+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:37.912031+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:46:56.435428+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |