rh_medium

CultureMech:001134 bacterial COMPLEX SOLID_AGAR
Original name: RH MEDIUM

Overview

External ontology
mediadive.medium:1652 (source link unavailable) — RH MEDIUM
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1652.pdf

Composition

flowchart LR
    CultureMech_001134["rh_medium"]
    ing0_L_Proline["L-Proline"]
    CultureMech_001134 --> ing0_L_Proline
    CHEBI_17203["CHEBI:17203"]
    ing0_L_Proline -.-> CHEBI_17203
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_001134 --> ing1_Yeast_extract
    ing2_Peptone["Peptone"]
    CultureMech_001134 --> ing2_Peptone
    ing3_Casamino_acids["Casamino acids"]
    CultureMech_001134 --> ing3_Casamino_acids
    ing4_Trehalose["Trehalose"]
    CultureMech_001134 --> ing4_Trehalose
    CHEBI_27082["CHEBI:27082"]
    ing4_Trehalose -.-> CHEBI_27082
    ing5_Glucose["Glucose"]
    CultureMech_001134 --> ing5_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing5_Glucose -.-> CHEBI_17234
    ing6_Starch["Starch"]
    CultureMech_001134 --> ing6_Starch
    CHEBI_28017["CHEBI:28017"]
    ing6_Starch -.-> CHEBI_28017
    ing7_Na_pyruvate["Na-pyruvate"]
    CultureMech_001134 --> ing7_Na_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing7_Na_pyruvate -.-> CHEBI_50144
    ing8_K2HPO4["K2HPO4"]
    CultureMech_001134 --> ing8_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing8_K2HPO4 -.-> CHEBI_131527
    ing9_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001134 --> ing9_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing9_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing10_KNO3["KNO3"]
    CultureMech_001134 --> ing10_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing10_KNO3 -.-> CHEBI_63043
    ing11_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001134 --> ing11_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing11_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing12_Agar["Agar"]
    CultureMech_001134 --> ing12_Agar
    CHEBI_2509["CHEBI:2509"]
    ing12_Agar -.-> CHEBI_2509

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 L-Proline
C5H9NO2 115.132 g/mol
0.5 G_PER_L CHEBI:17203
L-proline
2 Yeast extract 4 G_PER_L
3 Peptone 0.5 G_PER_L
4 Casamino acids 0.5 G_PER_L
5 Trehalose
C12H22O11 342.297 g/mol
1 G_PER_L CHEBI:27082
trehalose
6 Glucose
C6H12O6 180.156 g/mol
0.5 G_PER_L CHEBI:17234
glucose
7 Starch 0.5 G_PER_L CHEBI:28017
starch
8 Na-pyruvate
C3H3O3.Na 110.044 g/mol
0.3 G_PER_L CHEBI:50144
sodium pyruvate
9 K2HPO4
HO4P.2K 174.174 g/mol
0.3 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
10 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
11 KNO3
K.NO3 101.102 g/mol
0.5 G_PER_L CHEBI:63043
potassium nitrate
12 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.01 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
13 Agar 15 G_PER_L CHEBI:2509
agar

Preparation

  1. Final pH 7.2;
  2. And autoclave for 15 min at 121°C.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.132030Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1652
2026-01-28T19:48:19.562257Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:01.512355Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001134
2026-03-17T06:30:07.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:13.238255+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "RH MEDIUM" to "rh_medium" for consistent matching
2026-06-05T07:41:44.195765+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:50.185165+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:37:35.794959+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:10.902889+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T13:57:59.694168+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.